PXD018298

PXD018298 is an original dataset announced via ProteomeXchange.

Title	The degradome of the human intervertebral disc
Description	The intervertebral disc (IVD) is a joint in the spine that facilitates daily physical activity, comprising of the central nucleus pulposus (NP), surrounded by the annulus fibrosus (AF) and sandwiched between two cartilage endplates that function together as a unit. Changes to the IVD occur with aging, most drastically in the NP where it experiences dehydration and loss of cellularity, directly impacting on the integrity of the biomechanical functions of the IVD. We were interested in examining the types of degraded proteins in young and aged disc samples to further understand the protein turnover in aging and homeostasis. We analysed the degradome for degraded proteins using terminal amine isotopic labeling of substrates (TAILS) Clinical specimens from spine surgeries for scoliosis (young samples, n=3) or degeneration (aged samples, n=3) were used in this study.
HostingRepository	PRIDE
AnnounceDate	2021-01-29
AnnouncementXML	Submission_2021-01-29_02:42:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vivian Tam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	impact

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-31 01:38:00	ID requested
⏵ 1	2021-01-29 02:42:03	announced

Tam V, Chen P, Yee A, Solis N, Klein T, Kudelko M, Sharma R, Chan WC, Overall CM, Haglund L, Sham PC, Cheah KSE, Chan D, DIPPER, a spatiotemporal proteomics atlas of human intervertebral discs for exploring ageing and degeneration dynamics. Elife, 9():(2020) [pubmed]

submitter keyword: Intervertebral disc, proteomics, aging, proteomic architecture, degradome, dynamic proteome

Danny Chan
contact affiliation	The University of Hong Kong
contact email	chand@hku.hk
lab head
Vivian Tam
contact affiliation	The University of Hong Kong
contact email	viv.tam@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018298
     1. Label: PRIDE project
     2. Name: The degradome of the human intervertebral disc