PXD018297

PXD018297 is an original dataset announced via ProteomeXchange.

Title	Lon protease is important for growth under stressful conditions and pathogenicity of the phytopathogen, bacterium Dickeya solani.
Description	The Lon protein is a protease implicated in virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria include important potato pathogens, with the most aggressive species, D. solani. To determine the importance of Lon for pathogenicity and response to stress conditions of bacteria, we constructed a D. solani Δlon strain. The mutant bacteria showed increased sensitivity to certain stress conditions, in particular osmotic and high-temperature stresses. Furthermore, qPCR analysis showed an increased expression of the lon gene in D. solani under these conditions. The deletion of the lon gene resulted in decreased motility, lower activity of secreted pectinolytic enzymes and finally delayed onset of blackleg symptoms in the potato plants. In the Δlon cells, the altered levels of several proteins, including virulence factors and proteins associated with virulence, were detected by means of MS-SWATCH analysis. These included components of the type III secretion system and proteins involved in bacterial motility. Our results indicate that Lon protease is important for D. solani to withstand stressful conditions and effectively invade the potato plant.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_23:41:35.906.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Czaplewska
SpeciesList	scientific name: Dickeya solani; NCBI TaxID: 1089444;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-31 01:22:59	ID requested
⏵ 1	2021-09-08 23:41:36	announced

Figaj D, Czaplewska P, Przepi, ó, ra T, Ambroziak P, Potrykus M, Skorko-Glonek J, . Int J Mol Sci, 21(10):(2020) [pubmed]

submitter keyword: Dickeya solani, LC-MS/MS, SWATH

Paulina Czaplewska
contact affiliation	Intercollegiate FAculty of Biotechnology University of Gdansk and Medical University of Gdansk
contact email	pczaplewska@gmail.com
lab head
Paulina Czaplewska
contact affiliation	University of Gdansk
contact email	pczaplewska@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018297
     1. Label: PRIDE project
     2. Name: Lon protease is important for growth under stressful conditions and pathogenicity of the phytopathogen, bacterium Dickeya solani.