PXD018294

PXD018294 is an original dataset announced via ProteomeXchange.

Title	Characterization of NEK10 proteomic interactions under genotoxic stress
Description	Genetic studies in Aspergillus nidulans identified a family of protein kinases (NIMA- Never in mitosis gene A) crucial for cell cycle progression in fungus. Highly conserved in mammals, various members of NEKs (Nima-related kinases) cross-function in signaling networks depicted in at least one of the following main biological processes: mitotic events, DNA damage repair and response and in cilliagenesis. Recent studies have shown new functions for NEK1, 2, 4 and 5 in mitochondria respiration, apoptosis and protein complexes. NEK10 is the least studied member of the Neks, nevertheless NEK10 has been reported in Neks recurrent functions: G2/M control, centrosomes and cilliagenesis. Besides, the downregulation of Nek10 was associated with poor prognosis and aggressive breast tumors. Given that Neks function relies on protein signaling networks, we investigated Nek10 protein interacting partners and Nek10´s biological roles through IP-MS/MS. Our mass spectrometry data identified new interactions for Nek10 proteomics including mitochondria functional genes: SIRT3, ATAD3A, ATAD3B and OAT. The number of mitochondrial partners was augmented more than 3-fold after zeocin treatment: FKBP4, TXN, PFDN2, ATAD3B, MRPL12, ATP5J, DUT, YWHAE, CS, SIRT3, HSPA9, PDHB, GLUD1, DDX3X, and APEX1. We focused on exerting Nek10 function on the mitochondria and we first confirmed Nek10 localization in the mitochondria and its interaction with mitochondria proteins. To extend our understanding in Nek10 roles, we depleted Nek10 in mammalian cell lines further denoting mitochondria-related phenotypes such as inhibition of mitochondrial respiration, loss of mtDNA amplification and defects on mitochondria morphology. Our proteomics and functional data contributes to the first evidences of Nek10 role in mitochondria and our overall work drive Nek10 unknown roads towards the avenues of modulating mechanisms of diseases.
HostingRepository	PRIDE
AnnounceDate	2020-05-06
AnnouncementXML	Submission_2020-05-05_22:36:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PRISCILA SLEPICKA
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 23:35:19	ID requested
⏵ 1	2020-05-05 22:36:57	announced

Peres de Oliveira A, Basei FL, Slepicka PF, de Castro Ferezin C, Melo-Hanchuk TD, de Souza EE, Lima TI, Dos Santos VT, Mendes D, Silveira LR, Menck CFM, Kobarg J, NEK10 interactome and depletion reveal new roles in mitochondria. Proteome Sci, 18():4(2020) [pubmed]

submitter keyword: Nek10, Immunoprecipitation, LC-MS/MS, Mitochondria

Jörg Kobarg
contact affiliation	Laboratory of Signaling Mechanisms -LMS Institute of Biology, Department of Biochemistry and Tissue Biology Faculty of Pharmaceutical Sciences-FCF University of Campinas- UNICAMP
contact email	jorgkoba@unicamp.br
lab head
PRISCILA SLEPICKA
contact affiliation	LNBio
contact email	pfslepicka@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018294
     1. Label: PRIDE project
     2. Name: Characterization of NEK10 proteomic interactions under genotoxic stress