PXD018291

PXD018291 is an original dataset announced via ProteomeXchange.

Title	A decision tree searching strategy to boost the identification of cross-linked peptides
Description	We describe an efficient decision tree searching strategy (DTSS) to boost the identification of cross-linked peptides. The DTSS approach allows the identification of a wealth of complementary information to facilitate the construction of more protein-protein interaction networks for human cell lysate, which was tested by the use of a recently reported cross-linking dataset (ACS Cent. Sci. 2019, 5, 1514−1522). A variant of the PhoX-linker, named pDSPE was synthesized and applied to cross-link E.coli cell lysate to demonstrate that the acquisition of doubly charged ions can significantly improve identifica-tion results. The method can be seamlessly integrated to other search engines to maximize the number of identified cross-links
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:15:32.731.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018291
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wenzhang Chen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Biotin:Sigma-B1267; Carbamidomethyl
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 15:31:26	ID requested
⏵ 1	2024-10-08 11:15:42	announced

10.1021/acs.analchem.0c00452;

Huang R, Gao X, Xu Z, Zhu W, Wei D, Jiang B, Chen H, Chen W, Decision Tree Searching Strategy to Boost the Identification of Cross-Linked Peptides. Anal Chem, 92(20):13702-13710(2020) [pubmed]

10.6019/PXD018291;

submitter keyword: XLINK-MS, data-analysis

wenzhang chen
contact affiliation	SIAIS, ShanghaiTech University
contact email	chenwzh1@shanghaitech.edu.cn
lab head
Wenzhang Chen
contact affiliation	Shanghaitech University
contact email	chenwzh1@shanghaitech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD018291

PRIDE project URI

• PRIDE
  1. PXD018291
     1. Label: PRIDE project
     2. Name: A decision tree searching strategy to boost the identification of cross-linked peptides