PXD018290

PXD018290 is an original dataset announced via ProteomeXchange.

Title	Human melanoma MeWo cell phosphopeptide enriched Orbitrap MS
Description	Our newly developed high-throughput screening assay of bioactive compounds discovered a cellular phosphatase, calcineurin, was critical in the regulation of Varicella-Zoster Virus (VZV) glycoprotein complex gB/gH-gL mediated cell fusion. To better understand how calcineurin regulates fusion, the potential substrates for calcineurin phosphatase were investigated in human melanoma MeWo cells upon treatment with specific calcineurin phosphatase inhibitor, pimecrolimus. Phosphopeptide enrichment and Orbitrap mass spectrometry analysis identified seven proteins that were exclusively phosphorylated in MeWo cells treated with pimecrolimus compared to DMSO vehicle control, including two known calcineurin substrates. The other five proteins were novel targets for calcineurin dephosphorylation. These data implicated that one or more of these seven proteins in the calcineurin mediated regulation of VZV gB/gH-gL mediated cell fusion.
HostingRepository	PRIDE
AnnounceDate	2020-10-23
AnnouncementXML	Submission_2020-10-22_22:48:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018290
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Momei Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; Oxidation; acetylated residue; deaminated residue; Gln->pyro-Glu; Phospho; Propionamide; S-carboxamidoethyl-L-cysteine; Glu->pyro-Glu; Deamidated; Dioxidation; Acetyl; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 15:30:47	ID requested
⏵ 1	2020-10-22 22:48:39	announced

Dataset with its publication pending

submitter keyword: Human, Melanoma, Phosphopeptide, MS

Ann Arvin
contact affiliation	Department of Pediatrics, School of Medicine, Stanford University
contact email	arvinam@stanford.edu
lab head
Momei Zhou
contact affiliation	Stanford University
contact email	mzhou6@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD018290

PRIDE project URI

• PRIDE
  1. PXD018290
     1. Label: PRIDE project
     2. Name: Human melanoma MeWo cell phosphopeptide enriched Orbitrap MS