PXD018273 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics |
Description | IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple and complex protein samples prior phosphopeptide sequencing and characterization by mass spectrometry (LC-MS/MS). We optimized sample-loading conditions to increase phosphopeptide recovery for Zr-IMAC, Ti-IMAC and TiO2 based workflows. Furthermore, optimized Zr-IMAC and Ti-IMAC magnetic microsphere format were compared to HPLC-based Fe(III)-IMAC for same sample amount (200 ug). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:15:41.564.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignacio Arribas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-30 08:26:11 | ID requested | |
1 | 2020-11-26 02:30:04 | announced | |
⏵ 2 | 2024-10-22 05:15:43 | announced | 2024-10-22: Updated project metadata. |
Publication List
Arribas Diez I, Govender I, Naicker P, Stoychev S, Jordaan J, Jensen ON, Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment. J Proteome Res, 20(1):453-462(2021) [pubmed] |
10.1021/acs.jproteome.0c00508; |
Keyword List
submitter keyword: affinity enrichment, MOAC,IMAC, mass spectrometry, protein phosphorylation, functional proteomics, cell signalling, magnetic particles |
Contact List
Ole N. Jensen |
contact affiliation | Job title:Professor Department:Institut for Biokemi og Molekylær Biologi Company:Syddansk Universitet Location: Odense, Denmark |
contact email | jenseno@bmb.sdu.dk |
lab head | |
Ignacio Arribas |
contact affiliation | University of Southern Denmark |
contact email | sabirra92@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018273
- Label: PRIDE project
- Name: Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics