PXD018273

PXD018273 is an original dataset announced via ProteomeXchange.

Title	Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
Description	IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple and complex protein samples prior phosphopeptide sequencing and characterization by mass spectrometry (LC-MS/MS). We optimized sample-loading conditions to increase phosphopeptide recovery for Zr-IMAC, Ti-IMAC and TiO2 based workflows. Furthermore, optimized Zr-IMAC and Ti-IMAC magnetic microsphere format were compared to HPLC-based Fe(III)-IMAC for same sample amount (200 ug).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:41.564.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignacio Arribas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 08:26:11	ID requested
1	2020-11-26 02:30:04	announced
⏵ 2	2024-10-22 05:15:43	announced	2024-10-22: Updated project metadata.

Arribas Diez I, Govender I, Naicker P, Stoychev S, Jordaan J, Jensen ON, Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment. J Proteome Res, 20(1):453-462(2021) [pubmed]

10.1021/acs.jproteome.0c00508;

submitter keyword: affinity enrichment, MOAC,IMAC, mass spectrometry, protein phosphorylation, functional proteomics, cell signalling, magnetic particles

Ole N. Jensen
contact affiliation	Job title:Professor Department:Institut for Biokemi og Molekylær Biologi Company:Syddansk Universitet Location: Odense, Denmark
contact email	jenseno@bmb.sdu.dk
lab head
Ignacio Arribas
contact affiliation	University of Southern Denmark
contact email	sabirra92@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD018273

PRIDE project URI

• PRIDE
  1. PXD018273
     1. Label: PRIDE project
     2. Name: Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics