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PXD018273

PXD018273 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleZirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
DescriptionIMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple and complex protein samples prior phosphopeptide sequencing and characterization by mass spectrometry (LC-MS/MS). We optimized sample-loading conditions to increase phosphopeptide recovery for Zr-IMAC, Ti-IMAC and TiO2 based workflows. Furthermore, optimized Zr-IMAC and Ti-IMAC magnetic microsphere format were compared to HPLC-based Fe(III)-IMAC for same sample amount (200 ug).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:15:41.564.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIgnacio Arribas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; TripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-30 08:26:11ID requested
12020-11-26 02:30:04announced
22024-10-22 05:15:43announced2024-10-22: Updated project metadata.
Publication List
Arribas Diez I, Govender I, Naicker P, Stoychev S, Jordaan J, Jensen ON, Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment. J Proteome Res, 20(1):453-462(2021) [pubmed]
10.1021/acs.jproteome.0c00508;
Keyword List
submitter keyword: affinity enrichment, MOAC,IMAC, mass spectrometry, protein phosphorylation, functional proteomics, cell signalling, magnetic particles
Contact List
Ole N. Jensen
contact affiliationJob title:Professor Department:Institut for Biokemi og Molekylær Biologi Company:Syddansk Universitet Location: Odense, Denmark
contact emailjenseno@bmb.sdu.dk
lab head
Ignacio Arribas
contact affiliationUniversity of Southern Denmark
contact emailsabirra92@gmail.com
dataset submitter
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Dataset FTP location
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