PXD018264

PXD018264 is an original dataset announced via ProteomeXchange.

Title	Multi-protease analysis of Pleistocene bone proteomes
Description	Palaeoproteomics of skeletal tissues has the potential to provide new insights into the evolutionary relationships between globally distributed hominin fossils across the Pleistocene. The use of alternative or additional proteases has the potential to increase both proteome size and protein. However, the potential of alternative proteases beside trypsin remains unexplored systematically in palaeoproteomic studies. Here, we present a comparison of the bone proteome size and protein sequence recovery of six different proteases from four Pleistocene bone specimens. We observe that the majority of the preserved bone proteome is inaccessible to trypsin. We also note that for proteins recovered consistently across several proteases, protein sequence coverage can be increased significantly by combining peptide identifications from two or more proteases. Our results thereby demonstrate that the proteolysis of Pleistocene proteomes by several proteases has clear advantages when addressing evolutionary questions in palaeoproteomics.
HostingRepository	PRIDE
AnnounceDate	2020-10-13
AnnouncementXML	Submission_2020-10-12_23:29:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frido Welker
SpeciesList	scientific name: Homo; NCBI TaxID: 9605; scientific name: Equidae; NCBI TaxID: 9788;
ModificationList	monohydroxylated residue; monohydroxylated proline; deamidated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 03:42:49	ID requested
⏵ 1	2020-10-12 23:29:33	announced

Lanigan LT, Mackie M, Feine S, Hublin JJ, Schmitz RW, Wilcke A, Collins MJ, Cappellini E, Olsen JV, Taurozzi AJ, Welker F, Multi-protease analysis of Pleistocene bone proteomes. J Proteomics, 228():103889(2020) [pubmed]

submitter keyword: Bone, Pleistocene, proteases, Neanderthals

Enrico Cappellini
contact affiliation	Globe Institute, University of Copenhagen
contact email	ecappellini@sund.ku.dk
lab head
Frido Welker
contact affiliation	Postdoc Department of Biology, University of Copenhagen
contact email	frido.welker@palaeo.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD018264

PRIDE project URI

• PRIDE
  1. PXD018264
     1. Label: PRIDE project
     2. Name: Multi-protease analysis of Pleistocene bone proteomes