PXD018257

PXD018257 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of ARPE-19 cells in response to LPS
Description	Overwhelming and persistent inflammation of retinal pigment epithelium (RPE) induces destructive changes in retinal environment by invoking immune activation. In this study, we aimed to investigate RPE-specific biological and metabolic response against intense inflammation, and identify molecular characteristics determining pathological progression. Here, we performed quantitative analyses of proteome and phosphoproteome of lipopolysaccharide (LPS)-stimulated human derived RPE cell line ARPE-19 using the latest isobaric TMT labeling approach coupled with high-resolution mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_19:02:28.450.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dohyun Han
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 00:29:25	ID requested
⏵ 1	2021-09-08 19:02:29	announced

Song J, Han D, Lee H, Kim DJ, Cho JY, Park JH, Seok SH, A Comprehensive Proteomic and Phosphoproteomic Analysis of Retinal Pigment Epithelium Reveals Multiple Pathway Alterations in Response to the Inflammatory Stimuli. Int J Mol Sci, 21(9):(2020) [pubmed]

submitter keyword: ARPE-19, LPS, human

Dohyun Han
contact affiliation	Proteomics core facility, Biomedical Research Institute, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
lab head
Dohyun Han
contact affiliation	Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018257

PRIDE project URI

• PRIDE
  1. PXD018257
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of ARPE-19 cells in response to LPS