PXD018242
PXD018242 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human colon cancer HCT116 cell LC/MSMS |
| Description | Mitosis deregulation is a key event during carcinogenesis. Alternative splicing spectrum alteration plays a critical role during mitosis shift. However, the molecules responsible for dysregulated alternative splicing driving carcinogenetic mitosis remain poorly defined. Here, we demonstrate that cancer metastasis-associated antigen 1 (MTA1), a well-known oncogenic chromatin modifier, broadly interacts and co-expresses with RBPs across cancers, contributes to cancerous mitosis-related alternative splicing. Using developed fCLIP-seq technology, we show that MTA1 binds abundant transcripts, preferentially at splicing-responsible motifs, influencing both the abundance and alternative splicing (AS) of target transcripts. MTA1 is cytoplasmic and extrachromosomal at mitosis. Through the RNA-association activity, MTA1 regulates the mRNA level and guides the AS of a serious of mitosis regulators to control the mitosis. MTA1 deletion abrogated the dynamic AS switches of variants for ATRX and MYBL2 at mitotic stage, which are relevant to mitosis and tumorigenesis. MTA1 dysfunction causes a defective mitotic arrest, leading to aberrant chromosome segregation, and resultantly chromosomal instability (CIN) in cancer cells and tissues, which eventually contributes to tumorigenesis. Currently, little is known about the RNA splicing during mitosis, here, we present MTA1 as a pivotal RNA-binding protein, functioning to orchestrate the dynamic splicing of mitosis regulators linked to mitosis control in tumorigenesis. |
| HostingRepository | iProX |
| AnnounceDate | 2020-03-30 |
| AnnouncementXML | Submission_2023-03-01_18:44:29.259.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jian Liu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-03-29 17:39:47 | ID requested | |
| 1 | 2020-03-29 18:02:05 | announced | |
| ⏵ 2 | 2023-03-01 18:44:30 | announced | 2023-03-02: Update publication information. |
Publication List
| Liu J, Li C, Wang J, Xu D, Wang H, Wang T, Li L, Li H, Nan P, Zhang J, Wang Y, Huang C, Chen D, Zhang Y, Wen T, Zhan Q, Ma F, Qian H, Chromatin modifier MTA1 regulates mitotic transition and tumorigenesis by orchestrating mitotic mRNA processing. Nat Commun, 11(1):4455(2020) [pubmed] |
Keyword List
| submitter keyword: Human, colon cancer, HCT116, LC/MSMS |
Contact List
| Haili Qian | |
|---|---|
| contact affiliation | State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College |
| contact email | qianhaili001@163.com |
| lab head | |
| Jian Liu | |
| contact affiliation | Beijing Chao Yang Hospital, Capital Medical University |
| contact email | liujian2004811@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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