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PXD018225

PXD018225 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFacultative protein selenation controls redox sensitivity, adipose tissue thermogenesis and obesity
DescriptionOxidation of cysteines by reactive oxygen species (ROS) initiates thermogenesis in brown and beige adipose tissues. Cellular selenols, where sulfur is replaced with selenium, exhibit enhanced reactivity with ROS. Here we developed a mass spectrometric method to interrogate incorporation of selenols into proteins. Unexpectedly, this approach revealed facultative incorporation of selenium into proteins that lack canonical encoding for selenium-containing amino acids. Selenium was selectively incorporated into regulatory sites on key metabolic proteins, including as selenocysteine replacing cysteine at position 253 in UCP1. Remarkably, dietary selenium supplementation elevated facultative incorporation into UCP1, elevated energy expenditure through thermogenic adipose tissue, and protected against obesity. Together, these findings reveal the existence of facultative protein selenation, which correlates with impacts on thermogenic adipocyte function.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:28:10.014.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmittermark Jedrychowski
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListL-selenocysteine (Cys)
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-26 23:09:31ID requested
12021-09-09 01:06:41announced
22024-10-22 05:28:16announced2024-10-22: Updated project metadata.
Publication List
10.1073/pnas.2001387117;
Jedrychowski MP, Lu GZ, Szpyt J, Mariotti M, Garrity R, Paulo JA, Schweppe DK, Laznik-Bogoslavski D, Kazak L, Murphy MP, Gladyshev VN, Gygi SP, Chouchani ET, Spiegelman BM, Facultative protein selenation regulates redox sensitivity, adipose tissue thermogenesis, and obesity. Proc Natl Acad Sci U S A, 117(20):10789-10796(2020) [pubmed]
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Diabetes (B/D-HPP), Human Proteome Project
submitter keyword: UCP1, Thermogenesis,Selenium
Contact List
Edward Chouchani
contact affiliationDana Farber Assistant Professor
contact emailedwardT_chouchani@dfci.harvard.edu
lab head
mark Jedrychowski
contact affiliationHarvard Medical School
contact emailmark_jedrychowski@hms.harvard.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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