PXD018225 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Facultative protein selenation controls redox sensitivity, adipose tissue thermogenesis and obesity |
Description | Oxidation of cysteines by reactive oxygen species (ROS) initiates thermogenesis in brown and beige adipose tissues. Cellular selenols, where sulfur is replaced with selenium, exhibit enhanced reactivity with ROS. Here we developed a mass spectrometric method to interrogate incorporation of selenols into proteins. Unexpectedly, this approach revealed facultative incorporation of selenium into proteins that lack canonical encoding for selenium-containing amino acids. Selenium was selectively incorporated into regulatory sites on key metabolic proteins, including as selenocysteine replacing cysteine at position 253 in UCP1. Remarkably, dietary selenium supplementation elevated facultative incorporation into UCP1, elevated energy expenditure through thermogenic adipose tissue, and protected against obesity. Together, these findings reveal the existence of facultative protein selenation, which correlates with impacts on thermogenic adipocyte function. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:28:10.014.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | mark Jedrychowski |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | L-selenocysteine (Cys) |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-26 23:09:31 | ID requested | |
1 | 2021-09-09 01:06:41 | announced | |
⏵ 2 | 2024-10-22 05:28:16 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1073/pnas.2001387117; |
Jedrychowski MP, Lu GZ, Szpyt J, Mariotti M, Garrity R, Paulo JA, Schweppe DK, Laznik-Bogoslavski D, Kazak L, Murphy MP, Gladyshev VN, Gygi SP, Chouchani ET, Spiegelman BM, Facultative protein selenation regulates redox sensitivity, adipose tissue thermogenesis, and obesity. Proc Natl Acad Sci U S A, 117(20):10789-10796(2020) [pubmed] |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Diabetes (B/D-HPP), Human Proteome Project |
submitter keyword: UCP1, Thermogenesis,Selenium |
Contact List
Edward Chouchani |
contact affiliation | Dana Farber Assistant Professor |
contact email | edwardT_chouchani@dfci.harvard.edu |
lab head | |
mark Jedrychowski |
contact affiliation | Harvard Medical School |
contact email | mark_jedrychowski@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018225
- Label: PRIDE project
- Name: Facultative protein selenation controls redox sensitivity, adipose tissue thermogenesis and obesity