PXD018220

PXD018220 is an original dataset announced via ProteomeXchange.

Title	Analysis of histone PTMs in nascent chromatin in new and old histones upon DNA damage (CAL353, CAL358) using the NCC-SILAC approach
Description	Dynamic recycling and de novo deposition of histones are fundamental for chromatin restoration. Histone post-translational modifications (PTMs) are assumed to have a causal role in establishing distinct chromatin structures. However, it remains unknown how specific histone PTMs are regulated at broken replication fork. To get better insight into histone PTMs during DNA damage response, we combined NCC (Nascent chromatin capture) with SILAC-MS for identification of histone PTMs at replication forks upon CPT (camptothecin).
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:30:57.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-26 15:19:02	ID requested
⏵ 1	2021-03-04 12:30:59	announced

Nakamura K, Kustatscher G, Alabert C, H, ö, dl M, Forne I, V, ö, lker-Albert M, Satpathy S, Beyer TE, Mailand N, Choudhary C, Imhof A, Rappsilber J, Groth A, Proteome dynamics at broken replication forks reveal a distinct ATM-directed repair response suppressing DNA double-strand break ubiquitination. Mol Cell, 81(5):1084-1099.e6(2021) [pubmed]

submitter keyword: Histone PTMs, replication, camptothecin, NCC, SILAC

Prof. Anja Groth
contact affiliation	The Novo Nordisk Center for Protein Research (CPR), University of Copenhagen, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark
contact email	anja.groth@cpr.ku.dk
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD018220

PRIDE project URI

• PRIDE
  1. PXD018220
     1. Label: PRIDE project
     2. Name: Analysis of histone PTMs in nascent chromatin in new and old histones upon DNA damage (CAL353, CAL358) using the NCC-SILAC approach