PXD018211

PXD018211 is an original dataset announced via ProteomeXchange.

Title	Stoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system
Description	The multienzyme glycine cleavage system (GCS) converts glycine and tetrahydrofolate to the one-carbon compound 5,10-methylenetetrahydrofolate, which is of vital importance for most if not all organisms. Photorespiring plant mitochondria contain very high levels of GCS proteins organized as a fragile glycine decarboxylase complex (GDC). The aim of this study is to provide mass spectrometry-based stoichiometric data for the plant leaf GDC and examine whether complex formation could be a general property of the GCS in photosynthesizing organisms. To this end, we investigated the mitochondrial content, the stoichiometry and the isoprotein composition of the Arabidopsis thaliana leaf GDC in comparison with that of Pisum sativum using stable-isotope-labeled peptides (QconCAT and synthetic labeled peptides) and compared the results to label-free, Hi3 peptide quantification.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-04-26_22:52:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018211
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stefan Mikkat
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	Label:15N(1); Label:13C(6)15N(2); Label:15N(3); Oxidation; Label:15N(4); Carbamidomethyl; Label:15N(2); iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-26 01:59:25	ID requested
⏵ 1	2020-04-26 22:52:18	announced

Wittmi, ß M, Mikkat S, Hagemann M, Bauwe H, Stoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system. Plant J, 103(2):801-813(2020) [pubmed]

submitter keyword: Glycine decarboxylase complex, Stoichiometry, Arabidopsis, Pea, QconCAT, Quantification

Hermann Bauwe
contact affiliation	University of Rostock, Department of Plant Physiology, Albert-Einstein-Straße 3, D-18059 Rostock, Germany
contact email	hermann.bauwe@uni-rostock.de
lab head
Stefan Mikkat
contact affiliation	Core Facility Proteome Analysis, University Medicine Rostock, Schillingallee 69, 18057 Rostock
contact email	stefan.mikkat@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018211
     1. Label: PRIDE project
     2. Name: Stoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system