PXD018209

PXD018209 is an original dataset announced via ProteomeXchange.

Title	Unbiased proteomic profiling uncovers a targetable GNAS/PKA/PP2A axis in SCLC
Description	Using unbiased kinase profiling, we identified protein kinase A (PKA) as an active kinase in small cell lung cancer (SCLC). Inhibition of PKA activity genetically, or pharmacologically by activation of the PP2A phosphatase, inhibits SCLC expansion in culture and in vivo. Conversely, GNAS (G-protein α subunit), a PKA activator that is genetically activated in a small subset of human SCLC, promotes SCLC development. Phosphoproteomic analyses identified many new PKA substrates and mechanisms of action. In particular, PKA activity is required for the propagation of SCLC cancer stem cells in transplantation studies. Broad proteomic analysis of recalcitrant cancers has the potential to uncover novel targetable signaling networks, such as the GNAS/PKA/PP2A axis in SCLC.
HostingRepository	PRIDE
AnnounceDate	2020-06-17
AnnouncementXML	Submission_2020-06-16_23:30:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Gordan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-26 00:22:40	ID requested
⏵ 1	2020-06-16 23:30:12	announced

Coles GL, Cristea S, Webber JT, Levin RS, Moss SM, He A, Sangodkar J, Hwang YC, Arand J, Drainas AP, Mooney NA, Demeter J, Spradlin JN, Mauch B, Le V, Shue YT, Ko JH, Lee MC, Kong C, Nomura DK, Ohlmeyer M, Swaney DL, Krogan NJ, Jackson PK, Narla G, Gordan JD, Shokat KM, Sage J, Unbiased Proteomic Profiling Uncovers a Targetable GNAS/PKA/PP2A Axis in Small Cell Lung Cancer Stem Cells. Cancer Cell, 38(1):129-143.e7(2020) [pubmed]

submitter keyword: small cell lung cancer, kinase proteomics, analog specific, inhibitor beads

John Gordan
contact affiliation	Medicine, University of California San Francisco
contact email	John.Gordan@ucsf.edu
lab head
John Gordan
contact affiliation	University of California, San Francisco
contact email	john.gordan@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018209

PRIDE project URI

• PRIDE
  1. PXD018209
     1. Label: PRIDE project
     2. Name: Unbiased proteomic profiling uncovers a targetable GNAS/PKA/PP2A axis in SCLC