PXD018205

PXD018205 is an original dataset announced via ProteomeXchange.

Title	Restriction of essential amino acids dictates the systemic response to dietary protein dilution
Description	Dietary protein dilution (DPD) promotes metabolic remodelling and health but the precise nutritional components driving this response remain elusive. Here we demonstrate that dietary amino acids (AA) are sufficient and necessary to drive the response to DPD. In particular, the restriction of dietary essential AA (EAA) supply, but not non-EAA, drives the systemic metabolic response to total AA deprivation. Furthermore, systemic deprivation of Thr and Trp, independent of total AA supply, are both adequate and necessary to confer the systemic metabolic response to both diet, and genetic AA-transport loss, driven AA restriction. Thr is also potentially limiting in low-protein diet fed humans, and dietary Thr restriction (DTR) retarded the development of obesity-associated metabolic dysfunction in mice. Liver-derived fibroblast growth factor 21 was required for the metabolic remodelling with DTR. Strikingly, hepatocyte-selective establishment of Thr biosynthetic capacity reversed the systemic response to DTR. Taken together, our studies demonstrate that the restriction of EAA are sufficient and necessary to confer the systemic metabolic effects of DPD.
HostingRepository	PRIDE
AnnounceDate	2020-06-15
AnnouncementXML	Submission_2020-06-15_02:59:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cheng Huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-25 23:52:24	ID requested
⏵ 1	2020-06-15 02:59:16	announced

Yap YW, Rusu PM, Chan AY, Fam BC, Jungmann A, Solon-Biet SM, Barlow CK, Creek DJ, Huang C, Schittenhelm RB, Morgan B, Schmoll D, Kiens B, Piper MDW, Heikenw, ä, lder M, Simpson SJ, Br, ö, er S, Andrikopoulos S, M, ü, ller OJ, Rose AJ, Restriction of essential amino acids dictates the systemic metabolic response to dietary protein dilution. Nat Commun, 11(1):2894(2020) [pubmed]

submitter keyword: essential amino acids, Thr biosynthetic

Adam Rose
contact affiliation	1 Department of Biochemistry and Molecular Biology, Metabolism, Diabetes and Obesity Program, Biomedicine Discovery Institute, Monash University, Clayton 3800, Australia.
contact email	adam.rose@monash.edu
lab head
Cheng Huang
contact affiliation	Monash University
contact email	cheng.huang@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018205

PRIDE project URI

• PRIDE
  1. PXD018205
     1. Label: PRIDE project
     2. Name: Restriction of essential amino acids dictates the systemic response to dietary protein dilution