PXD018186

PXD018186 is an original dataset announced via ProteomeXchange.

Title	RAP-MS analysis of LncRNA BGL3 interacting proteins in cells
Description	Long non-coding RNAs (lncRNAs) are emerging regulators of genomic stability and human disease. However, the molecular mechanisms by which nuclear lncRNAs directly contribute to DNA damage responses remain largely unknown. Using RNA antisense purification coupled with quantitative mass spectrometry (RAP-qMS), we found that the lncRNA BGL3 binds to PARP1 and BARD1, exhibiting unexpected roles in homologous recombination. Mechanistically, BGL3 is recruited to DNA double-strand breaks (DSBs) by PARP1 at an early time point, which requires its interaction with the DNA-binding domain of PARP1. BGL3 also binds the C-terminal BRCT domain and an internal region (amino acids 127-424) of BARD1, which mediates interaction of the BRCA1/BARD1 complex with its binding partners such as HP1γ and RAD51, resulting in BRCA1/BARD1 retention at DSBs. Cells depleted for BGL3 displayed genomic instability and were sensitive to DNA-damaging reagents.
HostingRepository	PRIDE
AnnounceDate	2020-04-22
AnnouncementXML	Submission_2020-07-14_23:42:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaojie Mi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-25 00:20:20	ID requested
1	2020-04-21 22:11:54	announced
⏵ 2	2020-07-14 23:42:36	announced	2020-07-15: Updated publication reference for PubMed record(s): 32347575.

Hu Z, Mi S, Zhao T, Peng C, Peng Y, Chen L, Zhu W, Yao Y, Song Q, Li X, Li X, Jia C, Pei H, BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites. EMBO J, 39(12):e104133(2020) [pubmed]

submitter keyword: LncRNA BGL3 LC-MS

Huadong Pei
contact affiliation	Department of Biochemistry and Molecular Medicine, The George Washington University School of Medicine and Health Science, 2300 Eye Street, N.W., Washington, DC 20037, USA
contact email	huadongpei@gwu.edu
lab head
Shaojie Mi
contact affiliation	NCPSB
contact email	wh0419m@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD018186

PRIDE project URI

• PRIDE
  1. PXD018186
     1. Label: PRIDE project
     2. Name: RAP-MS analysis of LncRNA BGL3 interacting proteins in cells