PXD018179

PXD018179 is an original dataset announced via ProteomeXchange.

Title	Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots
Description	Background: Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields. Results: This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway. Conclusion: The results of this study improve our understanding of the mechanism of plant responses to Cd stress.
HostingRepository	PRIDE
AnnounceDate	2020-03-30
AnnouncementXML	Submission_2020-03-30_04:22:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	maling Jian
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-24 23:42:48	ID requested
⏵ 1	2020-03-30 04:22:37	announced

Dataset with its publication pending

submitter keyword: Cd stress

wheat roots

chemical forms

proteomics analysis

Lingjian Ma
contact affiliation	College of Horticulture, Northwest A&F University
contact email	malingjian@nwsuaf.edu.cn
lab head
maling Jian
contact affiliation	Northwest A&F University, College of Agronomy
contact email	malingjian@nwsuaf.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD018179

PRIDE project URI

• PRIDE
  1. PXD018179
     1. Label: PRIDE project
     2. Name: Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots