PXD018171

PXD018171 is an original dataset announced via ProteomeXchange.

Title	Quantitative secretome analysis using improved secretome-protein-enrichment-with-click-sugars method (iSPECS) establishes the cell type-resolved mouse brain glyco-secretome
Description	To understand how cells communicate with each other, it is essential to define the cellular secretome, a collection of proteins including soluble secreted, unconventionally secreted and proteolytically-shed proteins. Quantitative methodologies to decipher the secretome are challenging, due to the requirement of large cell numbers and abundant serum proteins that interfere with the detection of low-abundant cellular secretome proteins. Here, we miniaturized secretome analysis by developing the improved secretome-protein-enrichment-with-click-sugars method (iSPECS), which identifies the glyco-secretome. We applied this method to provide a cell type-resolved mouse brain glyco-secretome resource. Our data show that a surprisingly high number of secreted proteins are generated by ectodomain shedding in a cell type-specific manner. Two examples are neuronally secreted ADAM22 and CD200, which we identified as new substrates of the Alzheimer-linked protease BACE1. Taken together, iSPECS and the brain glyco-secretome resource can be exploited for a wide range of applications to study protein secretion and shedding.
HostingRepository	PRIDE
AnnounceDate	2020-09-25
AnnouncementXML	Submission_2020-09-25_03:45:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-24 23:09:50	ID requested
⏵ 1	2020-09-25 03:45:47	announced

T, ü, shaus J, M, ü, ller SA, Kataka ES, Zaucha J, Sebastian Monasor L, Su M, G, ü, ner G, Jocher G, Tahirovic S, Frishman D, Simons M, Lichtenthaler SF, An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome. EMBO J, 39(20):e105693(2020) [pubmed]

submitter keyword: Secretome, brain, Cerebrospinal Fluid, CSF, mouse, iSPECS, neuron, oligodendrocyte, astrocyte, microglia

Stefan F. Lichtenthaler
contact affiliation	Neuroproteomics DZNE Munich Feodor-Lynen-Str. 17 81377 Munich Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD018171

PRIDE project URI

• PRIDE
  1. PXD018171
     1. Label: PRIDE project
     2. Name: Quantitative secretome analysis using improved secretome-protein-enrichment-with-click-sugars method (iSPECS) establishes the cell type-resolved mouse brain glyco-secretome