PXD018168

PXD018168 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based analysis of Mycobacterial single-colony proteome
Description	Here as an attempt to explore bacterial single-colony proteomics, we describe a quantitative mass spectrometry-based protocol to isolate and analyse the proteome of a single mycobacterial colony from 7H10 media. Tryptic peptides are evaluated with high performance liquid chromatography coupled with a hybrid quadrupole mass filter Orbitrap analyser (Q Exactive) and raw data analysed using the MaxQuant Suite and downstream analysis using Perseus software . A total of 7805 unique peptides and 1387 proteins were identified
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:00.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	john Iradukunda
SpeciesList	scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-24 02:36:36	ID requested
1	2021-03-11 05:07:36	announced
⏵ 2	2024-10-22 05:20:00	announced	2024-10-22: Updated project metadata.

10.1007/978-1-0716-1178-4_11;

Iradukunda J, Ganief T, Blackburn JM, Soares NC, Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome. Methods Mol Biol, 2259():181-189(2021) [pubmed]

submitter keyword: protein extraction, Mycobacteria, bacterial proteome, Mass spectrometry,Single-colony, Proteomics

Jonathan Blackburn
contact affiliation	Department:Intergrated biomedical sciences,Division:Chemical biology,Institute of infectious diseases and Molecular medicine(IDM),Faculty of health science, University of Cape Town, South Africa
contact email	Jonathan.blackburn@uct.ac.za
lab head
john Iradukunda
contact affiliation	University of Cape Town
contact email	irdjoh001@myuct.ac.za
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD018168

PRIDE project URI

• PRIDE
  1. PXD018168
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based analysis of Mycobacterial single-colony proteome