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PXD018168

PXD018168 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry-based analysis of Mycobacterial single-colony proteome
DescriptionHere as an attempt to explore bacterial single-colony proteomics, we describe a quantitative mass spectrometry-based protocol to isolate and analyse the proteome of a single mycobacterial colony from 7H10 media. Tryptic peptides are evaluated with high performance liquid chromatography coupled with a hybrid quadrupole mass filter Orbitrap analyser (Q Exactive) and raw data analysed using the MaxQuant Suite and downstream analysis using Perseus software . A total of 7805 unique peptides and 1387 proteins were identified
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:20:00.055.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterjohn Iradukunda
SpeciesList scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-24 02:36:36ID requested
12021-03-11 05:07:36announced
22024-10-22 05:20:00announced2024-10-22: Updated project metadata.
Publication List
10.1007/978-1-0716-1178-4_11;
Iradukunda J, Ganief T, Blackburn JM, Soares NC, Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome. Methods Mol Biol, 2259():181-189(2021) [pubmed]
Keyword List
submitter keyword: protein extraction, Mycobacteria, bacterial proteome, Mass spectrometry,Single-colony, Proteomics
Contact List
Jonathan Blackburn
contact affiliationDepartment:Intergrated biomedical sciences,Division:Chemical biology,Institute of infectious diseases and Molecular medicine(IDM),Faculty of health science, University of Cape Town, South Africa
contact emailJonathan.blackburn@uct.ac.za
lab head
john Iradukunda
contact affiliationUniversity of Cape Town
contact emailirdjoh001@myuct.ac.za
dataset submitter
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Dataset FTP location
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PRIDE project URI
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