PXD018168 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry-based analysis of Mycobacterial single-colony proteome |
Description | Here as an attempt to explore bacterial single-colony proteomics, we describe a quantitative mass spectrometry-based protocol to isolate and analyse the proteome of a single mycobacterial colony from 7H10 media. Tryptic peptides are evaluated with high performance liquid chromatography coupled with a hybrid quadrupole mass filter Orbitrap analyser (Q Exactive) and raw data analysed using the MaxQuant Suite and downstream analysis using Perseus software . A total of 7805 unique peptides and 1387 proteins were identified |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:20:00.055.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | john Iradukunda |
SpeciesList | scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-24 02:36:36 | ID requested | |
1 | 2021-03-11 05:07:36 | announced | |
⏵ 2 | 2024-10-22 05:20:00 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1007/978-1-0716-1178-4_11; |
Iradukunda J, Ganief T, Blackburn JM, Soares NC, Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome. Methods Mol Biol, 2259():181-189(2021) [pubmed] |
Keyword List
submitter keyword: protein extraction, Mycobacteria, bacterial proteome, Mass spectrometry,Single-colony, Proteomics |
Contact List
Jonathan Blackburn |
contact affiliation | Department:Intergrated biomedical sciences,Division:Chemical biology,Institute of infectious diseases and Molecular medicine(IDM),Faculty of health science, University of Cape Town, South Africa |
contact email | Jonathan.blackburn@uct.ac.za |
lab head | |
john Iradukunda |
contact affiliation | University of Cape Town |
contact email | irdjoh001@myuct.ac.za |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD018168 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018168
- Label: PRIDE project
- Name: Mass spectrometry-based analysis of Mycobacterial single-colony proteome