PXD018167

PXD018167 is an original dataset announced via ProteomeXchange.

Title	Identification of O-glycosylation sites in Clostridium botulinum and Geobacillus kaustophilus flagellins
Description	In this study, we identified the O-glycosylation sites in recombinant Clostridium botulinum flagellin (CbFla) and Geobacillus kaustophilus flagellins, GkFlaA1 and GkFlaA2, expressed from the E. coli strains, EV136 and EV240 (K1:K12 strains engineered by Prof. Eric Vimr’s group to accumulate CMP-sialic acid in the cytosol), EV36 (wildtype K1:K12 strain), and BL21(DE3) (laboratory strain used for protein expression using T7 RNA polymerase). The recombinant flagellins were expressed with or without co-expression of motility associated factor (Maf) (flagellin nonulosonic acid glycosyltransferase) in these strains, purified and subjected to MS/MS analysis at Taplin Biological Mass Spectrometry facility.
HostingRepository	PRIDE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-07_00:02:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ramya Chakravarthy
SpeciesList	scientific name: Geobacillus kaustophilus HTA426; NCBI TaxID: 235909; scientific name: Clostridium botulinum F str. Langeland; NCBI TaxID: 441772;
ModificationList	N-acetylneuraminylated
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-24 02:20:55	ID requested
⏵ 1	2020-09-07 00:02:30	announced

Khairnar A, Sunsunwal S, Babu P, Ramya TNC, Novel serine/threonine-O-glycosylation with N-acetylneuraminic acid and 3-deoxy-D-manno-octulosonic acid by bacterial flagellin glycosyltransferases. Glycobiology, 31(3):288-306(2021) [pubmed]

submitter keyword: Neu5Ac, sialic acid, KDO, flagellin, Maf, nonulosonic acid, Gram positive, O-glycosylation, Clostridium botulinum, Geobacillus kaustophilus, LTQ Orbitrap Velos Pro ion-trap mass spectrometer

T.N.C. Ramya
contact affiliation	T.N.C. Ramya Principal Scientist CSIR-Institute of Microbial Technology Sector 39-A, Chandigarh 160036 INDIA Tel: 91-172-6665243; E-mail: ramya@imtech.res.in
contact email	ramya@imtech.res.in
lab head
Ramya Chakravarthy
contact affiliation	CSIR-Institute of Microbial Technology, Chandigarh
contact email	ramya@imtech.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018167
     1. Label: PRIDE project
     2. Name: Identification of O-glycosylation sites in Clostridium botulinum and Geobacillus kaustophilus flagellins