PXD018160

PXD018160 is an original dataset announced via ProteomeXchange.

Title	Plasma Protein Corona on Polystyrene Nanoparticles Facilitates their Transfer across the Human Placenta
Description	Nanoparticles exposed to biological fluids are rapidly surrounded by proteins. It is known that this formed protein corona influences the interplay of nanoparticles with cells or tissue barriers. In this study, we report the impact of a formed human plasma protein corona on the transfer of 80 nm polystyrene (PS) nanoparticles across the human placenta. We used the human ex-vivo placental perfusion model, as it reflects the intact and physiological tissue barrier between mother and unborn. Our results show an enhanced transfer of polystyrenes, exposed to human plasma, across the placenta compared to bovine serum albumin which served as control setting. We isolated nanoparticles before and after tissue exposure and analyzed their protein corona via shotgun proteomics and LC-MS/MS. The corona profile of particles that crossed the placenta highlighted several proteins as possible drivers for elevated tissue transfer. Subsequently two distinct proteins, human albumin and immunoglobulin G, were selected and incubated with the nanoparticles to form a sole protein corona. Strikingly, the protein corona formed by albumin induced significantly the transfer of polystyrenes across the tissue as compared to corona formed by immunoglobulins. To conclude, our study provides a comparative analysis between different formed protein coronas on nanoparticles and a corona dependent transfer behavior of polystyrenes across placental tissue. Our findings suggest that protein corona analyses of nanoparticles might help to understand better their properties at biological barriers.
HostingRepository	PRIDE
AnnounceDate	2020-08-19
AnnouncementXML	Submission_2020-09-15_23:12:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Darnhofer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-23 16:52:47	ID requested
1	2020-08-19 05:54:48	announced
⏵ 2	2020-09-15 23:14:53	announced	2020-09-16: Updated publication reference for PubMed record(s): 32907583.

Gruber MM, Hirschmugl B, Berger N, Holter M, Radulovi, ć S, Leitinger G, Liesinger L, Berghold A, Roblegg E, Birner-Gruenberger R, Bjelic-Radisic V, Wadsack C, Plasma proteins facilitates placental transfer of polystyrene particles. J Nanobiotechnology, 18(1):128(2020) [pubmed]

submitter keyword: nanoparticle, polystyrene, biocorona, dual ex vivo placental perfusion, human placenta, plasma proteins, transfer

Ruth Birner-Gruenberger
contact affiliation	Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology—TU Wien, Vienna, Austria; Institute of Pathology, Diagnostic and Research Center for Molecular BioMedicine, Medical University of Graz, Graz, Austria; Omics Center Graz, BioTechMed-Graz, Graz, Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Barbara Darnhofer
contact affiliation	Omics Center Graz
contact email	barbara.darnhofer@klinikum-graz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018160

PRIDE project URI

• PRIDE
  1. PXD018160
     1. Label: PRIDE project
     2. Name: Plasma Protein Corona on Polystyrene Nanoparticles Facilitates their Transfer across the Human Placenta