PXD018158

PXD018158 is an original dataset announced via ProteomeXchange.

Title	HEK293T cell degradome (pulse chase) time course (5h, 10, 15h) analysis upon Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)
Description	HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) time course experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 5h, 10h or 15h. Samples are Biotin-click, and streptavidin enriched, digested and labeled with TMTpro 16plex.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:54.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alban Ordureau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-23 16:51:50	ID requested
1	2020-04-29 22:20:34	announced
2	2020-07-05 22:46:46	announced	2020-07-06: Updated publication reference for PubMed record(s): 32612236.
⏵ 3	2024-10-22 05:02:55	announced	2024-10-22: Updated project metadata.

10.1038/s41586-020-2446-y;

An H, Ordureau A, K, ö, rner M, Paulo JA, Harper JW, Systematic quantitative analysis of ribosome inventory during nutrient stress. Nature, 583(7815):303-309(2020) [pubmed]

submitter keyword: Human, LC-MSMS, TMTpro, Azidohomoalanine (AHA)

Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA 02115 USA
contact email	wade_harper@hms.harvard.edu
lab head
Alban Ordureau
contact affiliation	Cell Biology Program, Sloan Kettering Institute, New York
contact email	alban_ordureau@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD018158

PRIDE project URI

• PRIDE
  1. PXD018158
     1. Label: PRIDE project
     2. Name: HEK293T cell degradome (pulse chase) time course (5h, 10, 15h) analysis upon Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)