PXD018157

PXD018157 is an original dataset announced via ProteomeXchange.

Title	Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry
Description	Neuropeptide Y (NPY) is a peptide widely distributed throughout the body that is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis and, as recently reported, the regulation of bone homeostasis. The Y1 receptors have been identified as major contributors to this latter biological effect. 5 - Carbamimidamido- 2 - (2,2 - diphenylacetamido) – N - [(4- hydroxyphenyl)methyl] pentanamide (BIBP 3226) is a selective NPY Y1 receptor antagonist with recognized potential as therapeutic agent in bone regeneration and its quantification in cellular internalization assays was aimed in the present work. Using high-performance liquid chromatography coupled to triple quadrupole-tandem mass spectrometry, BIBP 3226 determination was attained with a reversed phase column, isocratic elution, detection in positive ionization mode and data acquisition in selected reaction monitoring mode. Calibration curves were linear for concentrations ranging from 0.25 to 30 ng mL-1 with LOD and LOQ values as low as 0.1 and 0.3 pg in cell extracts and 16 and 48 pg in supernatant culture media, respectively. BIBP 3226 was successfully quantified in cell extracts and supernatants obtained from internalization assays using bone marrow cells (Wt C57/Bl6 mice) and also breast cancer cells (MCF7), which overexpress the NPY Y1 receptor. Therefore, the determined amount of BIBP 3226 was related to the levels of NPY Y1 receptor, demonstrating the method suitability to measure NPY Y1 receptor expression indirectly as an alternative to antibody-based fluorescence microscopy.
HostingRepository	PRIDE
AnnounceDate	2020-08-25
AnnouncementXML	Submission_2020-08-24_22:51:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Barreiros
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Shimadzu instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-23 16:51:05	ID requested
⏵ 1	2020-08-24 22:51:36	announced

Barreiros L, Silva EMP, Alencastre IS, Lamghari M, Segundo MA, Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry. Anal Bioanal Chem, 412(24):6625-6632(2020) [pubmed]

submitter keyword: Neuropeptide Y

Y1 receptor antagonist

BIBP 3226

Mass spectrometry

Receptor expression

Luisa Barreiros
contact affiliation	LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal
contact email	lbarreiros@ff.up.pt
lab head
Luisa Barreiros
contact affiliation	LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy of University of Porto, Porto, Portugal
contact email	lbarreiros@ff.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018157

PRIDE project URI

• PRIDE
  1. PXD018157
     1. Label: PRIDE project
     2. Name: Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry