PXD018157 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry |
Description | Neuropeptide Y (NPY) is a peptide widely distributed throughout the body that is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis and, as recently reported, the regulation of bone homeostasis. The Y1 receptors have been identified as major contributors to this latter biological effect. 5 - Carbamimidamido- 2 - (2,2 - diphenylacetamido) – N - [(4- hydroxyphenyl)methyl] pentanamide (BIBP 3226) is a selective NPY Y1 receptor antagonist with recognized potential as therapeutic agent in bone regeneration and its quantification in cellular internalization assays was aimed in the present work. Using high-performance liquid chromatography coupled to triple quadrupole-tandem mass spectrometry, BIBP 3226 determination was attained with a reversed phase column, isocratic elution, detection in positive ionization mode and data acquisition in selected reaction monitoring mode. Calibration curves were linear for concentrations ranging from 0.25 to 30 ng mL-1 with LOD and LOQ values as low as 0.1 and 0.3 pg in cell extracts and 16 and 48 pg in supernatant culture media, respectively. BIBP 3226 was successfully quantified in cell extracts and supernatants obtained from internalization assays using bone marrow cells (Wt C57/Bl6 mice) and also breast cancer cells (MCF7), which overexpress the NPY Y1 receptor. Therefore, the determined amount of BIBP 3226 was related to the levels of NPY Y1 receptor, demonstrating the method suitability to measure NPY Y1 receptor expression indirectly as an alternative to antibody-based fluorescence microscopy. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-25 |
AnnouncementXML | Submission_2020-08-24_22:51:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luisa Barreiros |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Shimadzu instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-23 16:51:05 | ID requested | |
⏵ 1 | 2020-08-24 22:51:36 | announced | |
Publication List
Barreiros L, Silva EMP, Alencastre IS, Lamghari M, Segundo MA, Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry. Anal Bioanal Chem, 412(24):6625-6632(2020) [pubmed] |
Keyword List
submitter keyword: Neuropeptide Y |
Y1 receptor antagonist |
BIBP 3226 |
Mass spectrometry |
Receptor expression |
Contact List
Luisa Barreiros |
contact affiliation | LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal |
contact email | lbarreiros@ff.up.pt |
lab head | |
Luisa Barreiros |
contact affiliation | LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy of University of Porto, Porto, Portugal |
contact email | lbarreiros@ff.up.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018157
- Label: PRIDE project
- Name: Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry