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PXD018157

PXD018157 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDetermination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry
DescriptionNeuropeptide Y (NPY) is a peptide widely distributed throughout the body that is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis and, as recently reported, the regulation of bone homeostasis. The Y1 receptors have been identified as major contributors to this latter biological effect. 5 - Carbamimidamido- 2 - (2,2 - diphenylacetamido) – N - [(4- hydroxyphenyl)methyl] pentanamide (BIBP 3226) is a selective NPY Y1 receptor antagonist with recognized potential as therapeutic agent in bone regeneration and its quantification in cellular internalization assays was aimed in the present work. Using high-performance liquid chromatography coupled to triple quadrupole-tandem mass spectrometry, BIBP 3226 determination was attained with a reversed phase column, isocratic elution, detection in positive ionization mode and data acquisition in selected reaction monitoring mode. Calibration curves were linear for concentrations ranging from 0.25 to 30 ng mL-1 with LOD and LOQ values as low as 0.1 and 0.3 pg in cell extracts and 16 and 48 pg in supernatant culture media, respectively. BIBP 3226 was successfully quantified in cell extracts and supernatants obtained from internalization assays using bone marrow cells (Wt C57/Bl6 mice) and also breast cancer cells (MCF7), which overexpress the NPY Y1 receptor. Therefore, the determined amount of BIBP 3226 was related to the levels of NPY Y1 receptor, demonstrating the method suitability to measure NPY Y1 receptor expression indirectly as an alternative to antibody-based fluorescence microscopy.
HostingRepositoryPRIDE
AnnounceDate2020-08-25
AnnouncementXMLSubmission_2020-08-24_22:51:35.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuisa Barreiros
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentShimadzu instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-23 16:51:05ID requested
12020-08-24 22:51:36announced
Publication List
Barreiros L, Silva EMP, Alencastre IS, Lamghari M, Segundo MA, Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry. Anal Bioanal Chem, 412(24):6625-6632(2020) [pubmed]
Keyword List
submitter keyword: Neuropeptide Y
Y1 receptor antagonist
BIBP 3226
Mass spectrometry
Receptor expression
Contact List
Luisa Barreiros
contact affiliationLAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal
contact emaillbarreiros@ff.up.pt
lab head
Luisa Barreiros
contact affiliationLAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy of University of Porto, Porto, Portugal
contact emaillbarreiros@ff.up.pt
dataset submitter
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