PXD018154
PXD018154 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | 20S proteasomes exported by malaria parasite promote its growth |
Description | Mature Red Blood Cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. While growing inside RBCs, Pf are known to secrete multipurpose extracellular vesicles (EVs), yet their influence on this parasite’s essential host cell, the RBC, remains unknown. Here we demonstrate that Pf parasites export within such EVs assembled and functional 20S proteasome complexes (EV-20S). The EV-20S proteasomes modulate the mechanical properties of naïve host RBCs by remodeling the cytoskeleton network. Furthermore, we identified four novel degradation targets of the exported 20S proteasome, the phosphorylated cytoskelatal proteins β-adducin, ankyrin-1, dematin and Epb4.1. Overall, our findings reveal a previously unknown 20S proteasome export mechanism of Pf-iRBCs, which prime naïve RBC by altering membrane stiffness, to facilitate malaria parasite growth. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:17:11.921.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamar Ziv |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-03-23 15:27:13 | ID requested | |
1 | 2021-01-03 23:42:20 | announced | |
2 | 2021-04-06 02:18:39 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33608523. |
⏵ 3 | 2024-10-22 05:17:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-021-21344-8; |
Dekel E, Yaffe D, Rosenhek-Goldian I, Ben-Nissan G, Ofir-Birin Y, Morandi MI, Ziv T, Sisquella X, Pimentel MA, Nebl T, Kapp E, Ohana Daniel Y, Karam PA, Alfandari D, Rotkopf R, Malihi S, Temin TB, Mullick D, Revach OY, Rudik A, Gov NS, Azuri I, Porat Z, Bergamaschi G, Sorkin R, Wuite GJL, Avinoam O, Carvalho TG, Cohen SR, Sharon M, Regev-Rudzki N, 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun, 12(1):1172(2021) [pubmed] |
Keyword List
submitter keyword: malaria, phosphorylation,Proteasome |
Contact List
Neta Regev-Rudzki | |
---|---|
contact affiliation | Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel |
contact email | neta.regev-rudzki@weizmann.ac.il |
lab head | |
Tamar Ziv | |
contact affiliation | Technion |
contact email | tamarz@tx.technion.ac.il |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD018154 |
PRIDE project URI |
Repository Record List
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