PXD018153

PXD018153 is an original dataset announced via ProteomeXchange.

Title	Proteome dynamics in persisting and resuscitating bacterial cells
Description	Bacterial persister cells become transiently tolerant to antibiotics by restraining their growth and metabolic activity. Detailed molecular characterization of bacterial persistence is hindered by low count of persisting cells and the need for their isolation. Here we used sustained addition of stable isotope-labeled lysine to selectively label the proteome of hipA-induced persisting and hipB-induced resuscitating E. coli cells in minimal medium after antibiotic treatment. Time-resolved, 24-hour measurement of label incorporation allowed detection of more than 500 newly synthetized proteins in persister cells, demonstrating low but widespread protein synthesis during persistence. Many essential proteins were newly synthesized; several ribosome-associated proteins showed unusually high synthesis levels and are involved in maintenance of persistence. At the onset of resuscitation, cells synthesized ABC transporters, restored translation machinery and resumed metabolism by inducing glycolysis and biosynthesis of amino acids. This dataset provides an unprecedented insight into the processes governing persistence and resuscitation of bacterial cells.
HostingRepository	PRIDE
AnnounceDate	2021-07-30
AnnouncementXML	Submission_2021-07-30_00:33:12.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-23 09:29:52	ID requested
⏵ 1	2021-07-30 00:33:12	announced

Dataset with its publication pending

submitter keyword: Escherichia coli, hipA, hipB, persistence, resuscitation

Boris Macek
contact affiliation	Quantitative Proteomics & Proteome Center Tuebingen Interfaculty Institute for Cell Biology Department of Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany
contact email	boris.macek@uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD018153

PRIDE project URI

• PRIDE
  1. PXD018153
     1. Label: PRIDE project
     2. Name: Proteome dynamics in persisting and resuscitating bacterial cells