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PXD018147 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLong noncoding RNA SAM promotes myoblast proliferation through stabilizing Sugt1 and facilitating kinetochore assembly
DescriptionLong non-coding RNAs (lncRNAs) are family of gene regulators but the functional study of lncRNAs in skeletal muscle satellite cells (SCs) remains at the infancy stage. Here we identify SAM (Sugt1 associated muscle) lncRNA (also known as Snhg15, (small nucleolar RNA host gene 15) that is enriched in the proliferating myoblast cells and down-regulated as the cells progressed to differentiation. Gain- or loss- of function of SAM in muscle SCs alters myogenic proliferation and differentiation. Global deletion of SAM based on the KO-first strategy has no overt effect on mice but impairs adult muscle regeneration following acute damage; the loss also exacerbates the chronic injury induced dystrophic phenotype in the mdx mouse model. Consistently, inducible deletion of SAM in adult SCs leads to deficiency in acute injury-induced muscle regeneration. Further examination of SCs revealed that SAM loss results in cell autonomous defect in proliferative expansion of myoblasts. Mechanistically, we find SAM interacts and stabilizes Sugt1 (suppressor of G2 allele of SKP1) protein, a co-chaperon protein key to kinetochore assembly during cell division. Loss of SAM or Sugt1 both cause disruptive kinetochore assembly and microtubule attachment in mitotic cells due to mis-localization of key components Dsn1 and Hec1 proteins. Altogether, our findings identify SAM as a regulator of SC proliferation through facilitating Sugt1 mediated kinetochore assembly during cell division.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuying LI
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-23 04:05:36ID requested
12020-05-18 01:26:51announced
22020-06-05 04:49:08announced2020-06-05: Updated publication reference for PubMed record(s): 32483152.
Publication List
Li Y, Yuan J, Chen F, Zhang S, Zhao Y, Chen X, Lu L, Zhou L, Chu CY, Sun H, Wang H, Long noncoding RNA SAM promotes myoblast proliferation through stabilizing Sugt1 and facilitating kinetochore assembly. Nat Commun, 11(1):2725(2020) [pubmed]
Keyword List
ProteomeXchange project tag: Skeletal Muscle Proteome (B/D-HPP)
submitter keyword: LC-MS
Contact List
Huating Wang
contact affiliationDept. of Orthopaedics and Traumatology The Chinese University of Hong Kong, Hong Kong, China
contact emailhuating.wang2011@gmail.com
lab head
Yuying LI
contact affiliationThe Chinese University of Hong Kong
contact emaillyy19870314@gmail.com
dataset submitter
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