PXD018140

PXD018140 is an original dataset announced via ProteomeXchange.

Title	Pharmacological and Phosphoproteomic Approaches to Roles of Protein Kinase C in Kappa Opioid Receptor-Mediated Effects in Mice
Description	KOR agonists possess adverse dysphoric and psychotomimetic effects, thus limiting their applications as anti-pruritic and non-addictive analgesic agents. Here, we showed that protein kinase C (PKC) inhibition preserved the KOR agonist induced beneficial antinociceptive and antipruritic effects, and attenuated the adverse condition placed aversion (CPA), sedation, and motor incoordination in mice. Using a large-scale mass spectrometry-based phosphoproteomics of KOR-mediated signaling in the brain, we observed at 5 minutes PKC-dependent modulation of G protein-coupled receptor kinases and Wnt pathways, and at 30 minutes modulation of stress signaling, cytoskeleton, mTOR signaling and receptor phosphorylation, including cannabinoid receptor CB1. We further demonstrated that inhibition of CB1 attenuated KOR-mediated CPA. Our results demonstrated the feasibility of in vivo biochemical dissection of signaling pathways that lead to side effects.
HostingRepository	PRIDE
AnnounceDate	2020-09-28
AnnouncementXML	Submission_2020-09-28_05:54:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeffrey Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-23 01:07:44	ID requested
⏵ 1	2020-09-28 05:54:16	announced

Liu JJ, Chiu YT, Chen C, Huang P, Mann M, Liu-Chen LY, Pharmacological and phosphoproteomic approaches to roles of protein kinase C in kappa opioid receptor-mediated effects in mice. Neuropharmacology, 181():108324(2020) [pubmed]

submitter keyword: Phosphoproteomic, brain, PKC, kappa opioid receptor

Matthias Mann
contact affiliation	Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
contact email	mmann@biochem.mpg.de
lab head
Jeffrey Liu
contact affiliation	Max Planck Institute of Biochemistry
contact email	jliu@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018140
     1. Label: PRIDE project
     2. Name: Pharmacological and Phosphoproteomic Approaches to Roles of Protein Kinase C in Kappa Opioid Receptor-Mediated Effects in Mice