PXD018129

PXD018129 is an original dataset announced via ProteomeXchange.

Title	NanoLC-MS/MS DDA Chroococcidiopsis thermalis PCC 7203 thylkoids WL vs. FRL
Description	Chroococcidiopsis thermalis is an extremophilic cyanobacterium that is adaptable to a widerange of environments including deserts, where it can maintain viability after desiccation. This species is also capable of photoautotrophic growth under far-red light (FRL, 750 nm) The proteomic comparison in this study, in conjunction with topographical imaging of thylakoid membranes by atomic force microscopy, supports earlier findings that FRL growth is accompanied by the synthesis of alternative photosynthesis-related proteins. Under white light (WL), a number of phycobilisome (PBS) and photosystem (PS) subunits were predominant at levels 2 - 14-fold greater compared to FRL growth. Two antenna family proteins were markedly more abundant under WL, with a fold-difference of approximately 80. While WL-associated proteins persisted at relatively low abundance under FRL, an exclusive set of PBS and PS subunit isoforms was expressed under FRL. These proteins were undetectable under WL, in many cases encoded in a discrete FRL-associated gene cluster. In line with the expected higher energy capture rate under WL, complexes associated with electron transport (eg. cytochrome b6f) and the ATP synthase were 1.2 – 3.4 times more abundant than under FRL.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_08:31:42.271.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Chroococcidiopsis thermalis PCC 7203; NCBI TaxID: 251229;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-20 14:08:10	ID requested
⏵ 1	2022-03-01 08:31:43	announced

MacGregor-Chatwin C, N, ü, rnberg DJ, Jackson PJ, Vasilev C, Hitchcock A, Ho MY, Shen G, Gisriel CJ, Wood WHJ, Mahbub M, Selinger VM, Johnson MP, Dickman MJ, Rutherford AW, Bryant DA, Hunter CN, Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation. Sci Adv, 8(6):eabj4437(2022) [pubmed]

submitter keyword: Chroococcidiopsis thermalis, thylkoid, photosystem, phycobilisome

ChristopherNeil Hunter
contact affiliation	Molecular Biology and Biotechnology, University of Sheffield
contact email	c.n.hunter@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	Department of Chemical and Biological Engineering, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018129
     1. Label: PRIDE project
     2. Name: NanoLC-MS/MS DDA Chroococcidiopsis thermalis PCC 7203 thylkoids WL vs. FRL