PXD018123 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | E.coli Ribosome protein composition |
Description | Ribosomes are essential macromolecular complexes conducting protein biosynthesis in all domains of life. Cells can have heterogeneous ribosomes, i.e. ribosomes with various ribosomal RNA (rRNA) and ribosomal protein (r-protein) composition. However, the functional importance of heterogeneous ribosomes has remained elusive. One of the possible sources for ribosome heterogeneity is provided by paralogous r-proteins. In E. coli, ribosomal protein bL31 has two paralogs: bL31A encoded by rpmE and bL31B encoded by ykgM. This study investigates phenotypic effects of these ribosomal protein paralogs using bacterial strains expressing only bL31A or bL31B. We show that bL31A confers higher fitness to E. coli under lower temperatures. In addition, bL31A and bL31B have different effects on translation reading frame maintenance and apparent translation processivity in vivo as demonstrated by dual luciferase assay. In general, this study demonstrates that ribosomal protein paralog composition (bL31A versus bL31B) can affect cell growth and translation outcome. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-20 |
AnnouncementXML | Submission_2020-10-20_01:05:32.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018123 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Kaspar Reier |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | Label:2H(4); Oxidation; Label:13C(6); monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap XL; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-20 07:00:09 | ID requested | |
⏵ 1 | 2020-10-20 01:05:33 | announced | |
Publication List
Lilleorg S, Reier K, Vol, รต, nkin P, Remme J, Liiv A, Phenotypic effects of paralogous ribosomal proteins bL31A and bL31B in E. coli. Sci Rep, 10(1):11682(2020) [pubmed] |
Keyword List
submitter keyword: Escherichia coli, Ribosome, LC-MS/MS |
Contact List
Tiina Tamm |
contact affiliation | Chair of Molecular Biology Institute of Cellular and Molecular Biology Estonia |
contact email | ttamm@ut.ee |
lab head | |
Kaspar Reier |
contact affiliation | University of Tatu |
contact email | kaspar.reier@ut.ee |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018123
- Label: PRIDE project
- Name: E.coli Ribosome protein composition