PXD018121

PXD018121 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Intracellular Freezing Survival
Description	Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has the extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While a few other organisms, all nematodes, have subsequently also been found to survive freezing in this manner, P. sp. DAW1 has so far shown the highest survival rates. In addition, P. sp. DAW1 is also, depending on the rate or extent of freezing, able to undergo cryoprotective dehydration. In this study, the proteome of P. sp DAW1 is explored, highlighting a number of differentially expressed proteins and pathways that appear to be involved in intracellular freezing. Among the strongest signals after being frozen is an upregulation of proteases and the downregulation of cytoskeletal and antioxidant activity, the latter possibly accumulated earlier much in the way the sugar trehalose is stored during acclimation.
HostingRepository	PRIDE
AnnounceDate	2020-05-01
AnnouncementXML	Submission_2020-05-01_06:03:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathryn Lilley
SpeciesList	scientific name: Panagrolaimus; NCBI TaxID: 55784;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-20 06:37:11	ID requested
⏵ 1	2020-05-01 06:03:36	announced

Dataset with its publication pending

submitter keyword: Panagrolaimus, intracellular freezing,cryoprotection, nematode

Michael A. Thorne
contact affiliation	British Antarctic Survey High Cross, Madingley Rd, Cambridge, CB3 0ET
contact email	mior@bas.ac.uk
lab head
Kathryn Lilley
contact affiliation	University of Cambridge
contact email	ksl23@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018121
     1. Label: PRIDE project
     2. Name: Proteomics of Intracellular Freezing Survival