PXD018115

PXD018115 is an original dataset announced via ProteomeXchange.

Title	Protein interaction partners of drosophila Unr identified by pull down of wild type and an interdomain mutant protein
Description	To identify an RNP interactome of Unr, V5 tagged wildtype protein and a mutant containing several interdomain contacts, that shows reduced protein expression were tansfected in SL2 cells after a siRNA knoch-down of the endogenous protein levels. After pulling down the transfected protein with an antibody against the V5-tag differences interacting proteins were identified using mass spectrometry. One set was done with an RNase treatment and one without, to identify interaction partners that rely on RNA binding. The lower expression level of the mutant protein allowed us to take this as a specific background control.
HostingRepository	PRIDE
AnnounceDate	2020-07-14
AnnouncementXML	Submission_2020-07-13_22:42:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-20 01:18:17	ID requested
⏵ 1	2020-07-13 22:42:38	announced

Dataset with its publication pending

submitter keyword: Unr, RBP, RNP, Pull-Down, interactome

Janosch Hennig
contact affiliation	Structural and Computational Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117, Heidelberg, Germany
contact email	janosch.hennig@embl.de
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018115

PRIDE project URI

• PRIDE
  1. PXD018115
     1. Label: PRIDE project
     2. Name: Protein interaction partners of drosophila Unr identified by pull down of wild type and an interdomain mutant protein