PXD018113

PXD018113 is an original dataset announced via ProteomeXchange.

Title	Antibody RING-Mediated Destruction (ARMeD) of endogenous proteins
Description	To understand the cellular function of a gene the encoding DNA or mRNA transcript can be manipulated and the consequences observed. However, these approaches do not have a direct effect on the protein product of the gene, which is either permanently abrogated or depleted at a rate defined by the half-life of the protein. Therefore, complete gene deletions and RNA interference of proteins with a very long half-life risk obscuring the direct consequences via compensatory or secondary cellular responses. We therefore sought to develop a single-component system that could induce the rapid degradation of the specific endogenous protein itself. A genetically introduced inducible construct of the RING domain of the ubiquitin E3 ligase RNF4 with a protein-specific camelid nanobody mediates destruction of the target by the ubiquitin proteasome system, a process we describe as Antibody RING-Mediated Destruction (ARMeD). The technique is acutely specific as we observed no off-target protein destruction. Furthermore, bacterially produced nanobody-RING fusion proteins electroporated into cells induce degradation of target within minutes. With the increasing availability of protein-specific nanobodies this method will allow the rapid and specific degradation of a wide range of endogenous proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:06:33.378.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mike Tatham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-19 16:40:52	ID requested
1	2020-05-29 00:31:51	announced
⏵ 2	2024-10-22 05:06:41	announced	2024-10-22: Updated project metadata.

Ibrahim AFM, Shen L, Tatham MH, Dickerson D, Prescott AR, Abidi N, Xirodimas DP, Hay RT, Antibody RING-Mediated Destruction of Endogenous Proteins. Mol Cell, 79(1):155-166.e9(2020) [pubmed]

10.1016/j.molcel.2020.04.032;

submitter keyword: RING Ubiquitin Nanobody Endogenous Degradation

Ronald T. Hay
contact affiliation	University of Dundee
contact email	r.t.hay@dundee.ac.uk
lab head
Mike Tatham
contact affiliation	University of Dundee
contact email	m.tatham@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018113

PRIDE project URI

• PRIDE
  1. PXD018113
     1. Label: PRIDE project
     2. Name: Antibody RING-Mediated Destruction (ARMeD) of endogenous proteins