PXD018112

PXD018112 is an original dataset announced via ProteomeXchange.

Title	The Aspergillus fumigatus secretome alters the proteome of Pseudomonas aeruginosa to stimulate bacterial growth: implications for co-infection
Description	Individuals with cystic fibrosis are susceptible to co-infection by Aspergillus fumigatus and Pseudomonas aeruginosa, however P. aeruginosa eventually predominates as the primary pathogen. Several factors are likely to facilitate P. aeruginosa colonization in the airways, including alterations to the microbial environment. In this study, significant growth proliferation was observed in P. aeruginosa when the bacteria were exposed to culture filtrates produced by A. fumigatus in a nitrate-rich, nutrient-poor liquid broth, Czapek-Dox. Proteomic analysis of the A. fumigatus culture filtrate identified a significant number of environment-altering proteases and peptidases, which may contribute to the growth promoting effect observed when P. aeruginosa is exposed to this culture filtrate. These findings offer insights into the determinants that contribute to P. aeruginosa proliferation in the presence of A. fumigatus.
HostingRepository	PRIDE
AnnounceDate	2020-07-22
AnnouncementXML	Submission_2020-07-22_01:46:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Aspergillus fumigatus Af293; NCBI TaxID: 330879;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-19 16:12:58	ID requested
⏵ 1	2020-07-22 01:46:32	announced

Margalit A, Carolan JC, Sheehan D, Kavanagh K, to Stimulate Bacterial Growth: Implications for Co-infection. Mol Cell Proteomics, 19(8):1346-1359(2020) [pubmed]

submitter keyword: Aspergillus fumigatus, culture filtrate, shotgun proteomics, LC-MS

Jim Carolan
contact affiliation	Applied Proteomics Lab, Department of Biology, Maynooth University, Co. Kildare, Ireland
contact email	james.c.carolan@gmail.com
lab head
James Carolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018112

PRIDE project URI

• PRIDE
  1. PXD018112
     1. Label: PRIDE project
     2. Name: The Aspergillus fumigatus secretome alters the proteome of Pseudomonas aeruginosa to stimulate bacterial growth: implications for co-infection