PXD018110

PXD018110 is an original dataset announced via ProteomeXchange.

Title	Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers
Description	Intracellular traffic between compartments of the secretory and endocytic pathways is mediated by vesicle-based carriers. The proteomes of carriers destined for many organelles are ill-defined because the vesicular intermediates are transient, low-abundance and difficult to purify. Here, we combine vesicle relocalisation with organelle proteomics and Bayesian analysis to define the content of different endosome-derived vesicles destined for the trans-Golgi network (TGN). The golgin coiled-coil proteins golgin-97 and GCC88, shown previously to capture endosome-derived vesicles at the TGN, were individually relocalised to mitochondria and the content of the subsequently re-routed vesicles was determined by organelle proteomics. Our findings reveal 45 integral and 51 peripheral membrane proteins re-routed by golgin-97, evidence for a distinct class of vesicles shared by golgin-97 and GCC88, and various cargoes specific to individual golgins. These results illustrate a general strategy for analysing intracellular sub-proteomes by combining acute cellular re-wiring with high-resolution spatial proteomics
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:02.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathryn Lilley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-19 16:09:55	ID requested
1	2020-12-01 08:59:20	announced
2	2023-05-14 14:04:56	announced	2023-05-14: Updated project metadata.
⏵ 3	2024-10-22 05:16:05	announced	2024-10-22: Updated project metadata.

10.1038/s41467-020-19840-4;

Shin JJH, Crook OM, Borgeaud AC, Cattin-Ortol, á J, Peak-Chew SY, Breckels LM, Gillingham AK, Chadwick J, Lilley KS, Munro S, Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers. Nat Commun, 11(1):5987(2020) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: LOPIT-DC, organell protein relocalization, Golgin, HeLa

Sean Munro
contact affiliation	MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH, UK
contact email	sean@mrc-lmb.cam.ac.uk
lab head
Kathryn Lilley
contact affiliation	University of Cambridge
contact email	ksl23@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018110

PRIDE project URI

• PRIDE
  1. PXD018110
     1. Label: PRIDE project
     2. Name: Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers