PXD018105

PXD018105 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SILAC-based quantitative MS analysis of proteome labeled by covalent hDM2 inhibitor
Description	SJSA1 cells grown in isotopically "light" or "heavy" medium were treated with Nutlin-3 (10 µM, 4 h). After removal of Nutlin-3 by washing, "light" amino acid-labeled cells were treated with DMSO or covalent hDM2 inhibitor (1 µM) in the presence of Nutlin-3 (10 µM) for 1 h, whereas "heavy" amino acid-labeled cells were treated with covalent hDM2 inhibitor (1 µM). Each cell population were lysed and combined in a 1:1 ratio. The covalent hDM2 inhibitor-modified proteins were conjugated to azide-PEG3-desthiobiotin and purified with Neutravidin beads. Enriched proteins were then subjected to in-gel trypsinization and the resultant peptides were analyzed by LC-MS/MS.
HostingRepository	jPOST
AnnounceDate	2021-03-19
AnnouncementXML	Submission_2022-09-18_03:03:39.257.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tsuyoshi Ueda
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-19 04:45:06	ID requested
1	2021-03-18 08:00:05	announced
⏵ 2	2022-09-18 03:03:39	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: human, SILAC

submitter keyword: human, SILAC

Contact List

Itaru Hamachi
lab head
Tsuyoshi Ueda
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000767/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000767/