PXD018100

PXD018100 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase
Description	Over-expression of Ppz1 is deleterious for yeast cells. Recent studies in our laboratory demonstrated that the over-expression of a catalytically impaired version of Ppz1 (Ppz1R451L) is not toxic, suggesting that the excess of Ppz1 phosphatase activity was responsible for toxicity, possibly due to changes in yeast phosphoproteome. Because the molecular basis of this toxicity are still unknown, a global yeast phosphoproteomic assay was designed to gain insight into this phenomenon. To identify revelant changes cuased by Ppz1 overexpression we have constructed a strain (ZCZ01) in which the PPZ1 promoter has been replaced by the GAL promotrer. Cell grown on raffinose were shifted to galactose and samples were taken after 30, 60, 120 and 240 min and changes in proteome and phosphoproteome were analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:15.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diego Velázquez
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-18 18:08:28	ID requested
1	2020-10-04 23:32:07	announced
2	2020-10-05 22:39:30	announced	2020-10-06: Updated project metadata.
3	2020-10-06 22:54:03	announced	2020-10-07: Updated project metadata.
4	2020-10-06 22:54:13	announced	2020-10-07: Updated project metadata.
⏵ 5	2024-10-22 05:13:20	announced	2024-10-22: Updated project metadata.

Vel, á, zquez D, Albacar M, Zhang C, Calaf, í C, L, ó, pez-Malo M, Torres-Torronteras J, Mart, í R, Kovalchuk SI, Pinson B, Jensen ON, Daignan-Fornier B, Casamayor A, Ari, ñ, o J, Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets. Sci Rep, 10(1):15613(2020) [pubmed]

10.1038/s41598-020-72391-y;

ProteomeXchange project tag: deep learning, benchmarking, machine learning

submitter keyword: Yeast, S. cerevisiae, phosphatase, antifungal target

Joaquin Ariño Carmona
contact affiliation	Head of the Biochemestry an molecular biology department
contact email	Joarin.Arino@uab.cat
lab head
Diego Velázquez
contact affiliation	Universitat Autonoma de Bercelona
contact email	Diego.Velazquez@uab.cat
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018100
     1. Label: PRIDE project
     2. Name: Proteomic and phosphoproteomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase