PXD018093

PXD018093 is an original dataset announced via ProteomeXchange.

Title	Multi-omics characterization of MEK inhibitor resistant pancreatic cancer based on a genetically engineered mouse model-derived in vitro system
Description	Tumor heterogeneity and therapy resistance are hallmarks of pancreatic ductal adenocarcinoma (PDAC). Emerging evidence supports treatment-induced resistance to be a multifactorial process mediated by cellular plasticity involving epigenetic regulation. Here, we used a multi-omics approach to analyze in detail molecular mechanisms underlying MEK inhibitor (MEKi) resistance. Therefore, we characterized different cell stages (parental, MEKi resistant, reverted after different passages of drug withdrawal) in primary cell lines derived from a genetic PDAC mouse model, thereby minimizing inter-individual heterogeneity that could distort genome-wide analyses.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:08:18.047.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018093
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kathrin Elisabeth Witzke
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	residues isobaric at 128.058578 Da; Oxidation; Carbamidomethyl; monohydroxylated residue; residues isobaric at 71.037114 Da; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-18 06:02:38	ID requested
⏵ 1	2024-01-26 06:08:18	announced

10.1186/s13148-024-01623-z;

10.6019/PXD018093;

Godfrey LK, Forster J, Liffers ST, Schr, ö, der C, K, ö, ster J, Henschel L, Ludwig KU, L, ä, hnemann D, Trajkovic-Arsic M, Behrens D, Scarpa A, Lawlor RT, Witzke KE, Sitek B, Johnsen SA, Rahmann S, Horsthemke B, Zeschnigk M, Siveke JT, Pancreatic cancer acquires resistance to MAPK pathway inhibition by clonal expansion and adaptive DNA hypermethylation. Clin Epigenetics, 16(1):13(2024) [pubmed]

submitter keyword: mouse model,PDAC, MEK inhibitor resistant, LC-MS/MS

Barbara Sitek
contact affiliation	Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany
contact email	barbara.sitek@rub.de
lab head
Kathrin Elisabeth Witzke
contact affiliation	Ruhr-University Bochum
contact email	kathrin.witzke@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD018093

PRIDE project URI

• PRIDE
  1. PXD018093
     1. Label: PRIDE project
     2. Name: Multi-omics characterization of MEK inhibitor resistant pancreatic cancer based on a genetically engineered mouse model-derived in vitro system