PXD018087

PXD018087 is an original dataset announced via ProteomeXchange.

Title	A rendezvous of two second messengers: The c-di-AMP receptor protein DarB controls (p)ppGpp synthesis in Bacillus subtilis
Description	DarB is a cyclic di-AMP binding protein consisting of two CBS (Cystathionine-beta synthase) domains. To reveal the global role of the second-messenger, understanding the function of the receptor proteins is fundamental. In order to do so, we aimed to identify potential binding partners of DarB in vitro with an unbiased protein pulldown experiment. In this study, we identified the (p)ppGpp synthetase/ hydrolase RelA as the binding partner of DarB and could confirm these findings with an in vivo interaction experiment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:43.300.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-18 02:20:08	ID requested
1	2021-01-11 23:57:49	announced
⏵ 2	2024-10-22 05:17:44	announced	2024-10-22: Updated project metadata.

10.1038/s41467-021-21306-0;

Kr, ü, ger L, Herzberg C, Wicke D, B, ä, hre H, Heidemann JL, Dickmanns A, Schmitt K, Ficner R, St, ü, lke J, A meet-up of two second messengers: the c-di-AMP receptor DarB controls (p)ppGpp synthesis in Bacillus subtilis. Nat Commun, 12(1):1210(2021) [pubmed]

submitter keyword: RelA,Bacillus subtilis, pull-down, second messengers, c-di-AMP receptor, DarB

Jörg Stülke
contact affiliation	Georg-August-University Göttingen, Department of General Microbiology, Institute of Microbiology and Genetics
contact email	jstuelk@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD018087

PRIDE project URI

• PRIDE
  1. PXD018087
     1. Label: PRIDE project
     2. Name: A rendezvous of two second messengers: The c-di-AMP receptor protein DarB controls (p)ppGpp synthesis in Bacillus subtilis