PXD018071

PXD018071 is an original dataset announced via ProteomeXchange.

Title	Biotinylated surfome profiling of A. fumigatus during early development
Description	Aspergillus fumigatus is one of the most common airborne fungi that cause invasive mycoses in immunocompromised patients and also allergic diseases in immunocompetent individuals. In both cases, the surface proteins mediate the first contact with the human immune system to evade immune responses or to induce hypersensitivity. The peptides exposed on the surface may be reasonable immunotherapy targets or may be used for diagnostic purposes. Several methods have been established to study the surface proteome (surfome) of A. fumigatus, like trypsin shaving, glucanase treatment, or formic acid extraction. Biotinylation coupled with LC-MS/MS identification of peptides is a particularly efficient method to identify the surface exposed regions of proteins that could mediate interaction with the host. After biotinylation of surface proteins during the germination process, we detected 314 surface proteins, including several well-known surface proteins (like RodA, CcpA, and DppV), allergens, heat shock proteins (Hsp), as well as some previously uncharacterized surface proteins. Using immunofluorescence microscopy, we confirmed the surface localization of several chaperone proteins. Collectively, our study generated a comprehensive data set of the A. fumigatus surfome. In addition, for many proteins it uncovers the regions that are exposed on the surface of spores or hyphae. These surface exposed regions could directly interact with host cells and may represent antigenic epitopes that induce protective immune responses.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:23.537.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Aspergillus fumigatus CEA10; NCBI TaxID: 505235;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-17 01:52:35	ID requested
1	2020-04-21 22:57:41	announced
2	2021-01-06 07:31:31	announced	2021-01-06: Updated publication reference for PubMed record(s): 32817453.
⏵ 3	2024-10-22 05:02:24	announced	2024-10-22: Updated project metadata.

10.1128/msphere.00535-20;

Jia LJ, Kr, ü, ger T, Blango MG, von Eggeling F, Kniemeyer O, Brakhage AA, Biotinylated Surfome Profiling Identifies Potential Biomarkers for Diagnosis and Therapy of Aspergillus fumigatus Infection. mSphere, 5(4):(2020) [pubmed]

submitter keyword: surface biotinylation,Aspergillus fumigatus, allergens, surfome, LC-MS/MS

Axel A. Brakhage
contact affiliation	Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Adolf-Reichwein-Str. 23, 07745, Jena, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD018071

PRIDE project URI

• PRIDE
  1. PXD018071
     1. Label: PRIDE project
     2. Name: Biotinylated surfome profiling of A. fumigatus during early development