PXD018066

PXD018066 is an original dataset announced via ProteomeXchange.

Title	On-chip sample preparation using a ChipFilter coupled to NanoLC-MS/MS for bottom-up proteomics
Description	Sample preparation is a crucial step in bottom-up proteomics. Analytical performances of bottom-up proteomics can be improved by the miniaturization of sample preparation steps. Many microfluidic devices are proposed in the field of proteomics. But many of them are not capable of handling complex sample and do not integrate the processing and digestion steps. We propose a ChipFilter Proteolysis (CFP) microfluidic device derived from the Filter Aided Sample Preparation FASP method for the miniaturization of protein processing and digestion steps in bottom-up proteomics. The microchip has two reaction chambers of 0.6 µL volume separated by a protein filtration membrane in regenerated cellulose. Cell lysis, protein concentration and rapid chemical and enzymatic treatment can be performed in our microfluidic device. Complex proteomic samples like yeast protein extract have already been analyzed with our microchip. Compared to the traditional FASP method, our microfluidic device offers a better proteome coverage with ten times less starting material and eight times quicker protocol.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:12.707.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Massamba Ndiaye
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-17 01:41:59	ID requested
1	2020-05-05 23:00:54	announced
⏵ 2	2024-10-22 05:03:13	announced	2024-10-22: Updated project metadata.

Ndiaye MM, Ta HP, Chiappetta G, Vinh J, On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics. J Proteome Res, 19(7):2654-2663(2020) [pubmed]

10.1021/acs.jproteome.9b00832;

submitter keyword: miniaturization,proteomics

Joëlle Vinh
contact affiliation	SMBP lab, CNRS, ESPCI PSL Paris, France (lab head)
contact email	joelle.vinh@espci.fr
lab head
Massamba Ndiaye
contact affiliation	PSL
contact email	massamba-mbake.ndiaye@espci.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD018066

PRIDE project URI

• PRIDE
  1. PXD018066
     1. Label: PRIDE project
     2. Name: On-chip sample preparation using a ChipFilter coupled to NanoLC-MS/MS for bottom-up proteomics