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PXD018066

PXD018066 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOn-chip sample preparation using a ChipFilter coupled to NanoLC-MS/MS for bottom-up proteomics
DescriptionSample preparation is a crucial step in bottom-up proteomics. Analytical performances of bottom-up proteomics can be improved by the miniaturization of sample preparation steps. Many microfluidic devices are proposed in the field of proteomics. But many of them are not capable of handling complex sample and do not integrate the processing and digestion steps. We propose a ChipFilter Proteolysis (CFP) microfluidic device derived from the Filter Aided Sample Preparation FASP method for the miniaturization of protein processing and digestion steps in bottom-up proteomics. The microchip has two reaction chambers of 0.6 µL volume separated by a protein filtration membrane in regenerated cellulose. Cell lysis, protein concentration and rapid chemical and enzymatic treatment can be performed in our microfluidic device. Complex proteomic samples like yeast protein extract have already been analyzed with our microchip. Compared to the traditional FASP method, our microfluidic device offers a better proteome coverage with ten times less starting material and eight times quicker protocol.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:03:12.707.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMassamba Ndiaye
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-17 01:41:59ID requested
12020-05-05 23:00:54announced
22024-10-22 05:03:13announced2024-10-22: Updated project metadata.
Publication List
Ndiaye MM, Ta HP, Chiappetta G, Vinh J, On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics. J Proteome Res, 19(7):2654-2663(2020) [pubmed]
10.1021/acs.jproteome.9b00832;
Keyword List
submitter keyword: miniaturization,proteomics
Contact List
Joëlle Vinh
contact affiliationSMBP lab, CNRS, ESPCI PSL Paris, France (lab head)
contact emailjoelle.vinh@espci.fr
lab head
Massamba Ndiaye
contact affiliationPSL
contact emailmassamba-mbake.ndiaye@espci.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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