PXD018061

PXD018061 is an original dataset announced via ProteomeXchange.

Title	Viral hijacking of the TENT4-ZCCHC14 complex protects viral RNAs via mixed tailing
Description	TENT4 enzymes generate “mixed tails” on mRNAs by adding adenosines with intermittent non-adenosine residues, which protect mRNAs from deadenylation. Here we discover the extensive mixed tailing in the transcripts of two distinct DNA viruses, hepatitis B virus (HBV) and human cytomegalovirus (HCMV), and their striking similarity in the mechanism to exploit the TENT4-ZCCHC14 complex. TAIL-seq analyses of HBV and HCMV RNAs revealed that TENT4A and TENT4B are responsible for the mixed tailing and protection of viral poly(A) tails. By fCLIP-seq on HBV-producing cells, we found that the HBV posttranscriptional regulatory element (PRE) is the primary site of the TENT4 interactome. A hairpin with a CNGGN-type pentaloop in the PRE is critical for TENT4-dependent regulation. Unexpectedly, HCMV also utilizes a similar pentaloop hairpin, an interesting example of convergent evolution. We further found that the CNGGN pentaloop is recognized by the SAM domain-containing ZCCHC14 protein which in turn recruits TENT4 to stabilize viral transcripts. Altogether, our study reveals the action mechanism of PRE that has been widely used to enhance gene expression, and introduces the TENT4-ZCCHC14 complex as the key host factor and a potential target for antiviral therapeutics.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-07-15_00:31:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018061
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Young-suk Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-16 16:38:51	ID requested
1	2020-04-27 00:13:47	announced
⏵ 2	2020-07-15 00:31:02	announced	2020-07-15: Updated publication reference for PubMed record(s): 32451488.

Kim D, Lee YS, Jung SJ, Yeo J, Seo JJ, Lee YY, Lim J, Chang H, Song J, Yang J, Kim JS, Jung G, Ahn K, Kim VN, Viral hijacking of the TENT4-ZCCHC14 complex protects viral RNAs via mixed tailing. Nat Struct Mol Biol, 27(6):581-588(2020) [pubmed]

submitter keyword: RNA modification, Viral RNA, RNA-Protein Interaction

V. Narry Kim
contact affiliation	Institute for Basic Science and Seoul National University
contact email	narrykim@snu.ac.kr
lab head
Young-suk Lee
contact affiliation	Institute of Basic Science
contact email	2yngskl@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018061
     1. Label: PRIDE project
     2. Name: Viral hijacking of the TENT4-ZCCHC14 complex protects viral RNAs via mixed tailing