PXD018052

PXD018052 is an original dataset announced via ProteomeXchange.

Title	The transcription factor TAL1 and the miR-17-92 cluster establish a regulatory loop
Description	A network of gene regulatory factors such as transcription factors and microRNAs establish and maintain the gene expression pattern during hematopoiesis. In this network transcription factors regulate each other and are involved in regulatory loops with microRNAs.The microRNA cluster miR-17-92 is located within the MIR17HG gene and encodes for six mature microRNAs. It is important for hematopoietic differentiation and plays a central role in malignant disease. However, the transcription factors downstream of miR-17-92 are largely elusive and the transcriptional regulation of miR-17-92 is not fully understood. Here we show that miR-17-92 forms a regulatory loop with the transcription factor TAL1. The miR-17-92 cluster inhibits expression of TAL1 and indirectly leads to decreased stability of the TAL1 transcriptional complex. We found that TAL1 and its heterodimerization partner E47 regulate miR-17-92 transcriptionally. Furthermore, miR-17-92 negatively influences erythroid differentiation, a process that depends on gene activation by the TAL1 complex. Our data give example of how transcription factor activity is fine-tuned during normal hematopoiesis. We postulate that disturbance of the regulatory loop between TAL1 and the miR-17-92 cluster could be an important step in cancer development and progression.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_11:40:26.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jörn Lausen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 05:14:34	ID requested
⏵ 1	2022-02-15 11:40:27	announced

Meyer A, Herkt S, Kunze-Schumacher H, Kohrs N, Ringleb J, Schneider L, Kuvardina ON, Oellerich T, H, ä, upl B, Krueger A, Seifried E, Bonig H, Lausen J, The transcription factor TAL1 and miR-17-92 create a regulatory loop in hematopoiesis. Sci Rep, 10(1):21438(2020) [pubmed]

submitter keyword: Human, blood, microRNA, miR-17-92, TAL1, hematopoiesis, Jurkat cells

Joern Lausen
contact affiliation	Universität Stuttgart Institut für Industrielle Genetik Abteilung Eukaryotengenetik Allmandring 31 70569 Stuttgart
contact email	joern.lausen@iig.uni-stuttgart.de
lab head
Jörn Lausen
contact affiliation	Universität Stuttgart Institut für Industrielle Genetik Abteilung Eukaryotengenetik Allmandring 31 70569 Stuttgart
contact email	joern.lausen@iig.uni-stuttgart.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018052

PRIDE project URI

• PRIDE
  1. PXD018052
     1. Label: PRIDE project
     2. Name: The transcription factor TAL1 and the miR-17-92 cluster establish a regulatory loop