PXD018049

PXD018049 is an original dataset announced via ProteomeXchange.

Title	Feedback regulation in EGFR signaling involves IRF2BP proteins and temporal control by (de)SUMOylation
Description	Molecular switches are essential modules in signaling networks by enabling a quick response to environmental changes. Here, we describe a role for small ubiquitin-like-modifier SUMO as a molecular switch in epidermal growth factor receptor (EGFR) signaling. Using quantitative mass spectrometry, we compared the endogenous SUMO-proteomes of Hela cells before and after EGF-stimulation. Thereby, we identified a small group of transcriptional repressors, including IRF2BP1, IRF2BP2 and IRF2BPL as novel players in EGFR signaling, which lost SUMO transiently within 15 min of EGF treatment. We found 38 genes whose expression within the first hour of EGF treatment was altered in cells expressing SUMOylation deficient IRF2BP1, including Dual specific phosphatase 1 (DUSP1), an important feedback regulator of EGFR signalling. We show that IRF2BP1 binds to the proximal promoter of DUSP1, whose activation requires IRF2BP1 deSUMOylation. Thus, the SUMO-dependent molecular switch of IRF2BP1 activity is important to finely tune EGFR signaling
HostingRepository	PRIDE
AnnounceDate	2021-04-14
AnnouncementXML	Submission_2021-04-13_22:47:29.074.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uwe PLessmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 05:10:50	ID requested
1	2021-04-13 05:12:59	announced
⏵ 2	2021-04-13 22:47:29	announced	2021-04-14: Updated project metadata.

Barysch SV, Stankovic-Valentin N, Miedema T, Karaca S, Doppel J, Nait Achour T, Vasudeva A, Wolf L, Sticht C, Urlaub H, Melchior F, Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling. EMBO Rep, 22(3):e49651(2021) [pubmed]

10.15252/EMBR.201949651;

submitter keyword: IRF2BP1, DUSP1, transcription, IRF2BP2,EGF, feedback inhibitor, EGFR, SUMO, regulation

Henning Urlaub
contact affiliation	Bioanalytical Mass Spectromerty Group Max Planck Institute for Biophysical Chemnistry Goettingen, Germany
contact email	hurlaub@gwdg.de
lab head
Uwe PLessmann
contact affiliation	Max Planck Intitute for biophysical Chemnistry
contact email	uplessm@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD018049

PRIDE project URI

• PRIDE
  1. PXD018049
     1. Label: PRIDE project
     2. Name: Feedback regulation in EGFR signaling involves IRF2BP proteins and temporal control by (de)SUMOylation