PXD018044

PXD018044 is an original dataset announced via ProteomeXchange.

Title	Epitope shaving and fungal immune evasion – culture supernatants
Description	The cell wall provides a major physical interface between fungal pathogens and their mammalian host. This extracellular armour is critical for fungal cell homeostasis and survival. Yet essential cell wall moieties, such as β-1,3-glucan, are recognised as pathogen-associated molecular patterns (PAMPs) that activate immune-mediated clearance mechanisms. We have reported that the opportunistic human fungal pathogen, Candida albicans, masks β-1,3-glucan following exposure to lactate, hypoxia or iron depletion. However, the precise mechanism(s) by which C. albicans masks β-1,3-glucan have remained obscure. Here, we performed proteomic analysis of supernatants harvested from C. albicans cells grown in hypoxia or lactate compared to glucose-grown controls to identify mechanisms driving β-1,3-glucan masking.
HostingRepository	PRIDE
AnnounceDate	2020-06-15
AnnouncementXML	Submission_2020-07-16_02:28:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Stead
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 02:48:58	ID requested
1	2020-06-15 03:08:00	announced
⏵ 2	2020-07-16 02:28:38	announced	2020-07-16: Updated publication reference for PubMed record(s): 32636248.

Childers DS, Avelar GM, Bain JM, Pradhan A, Larcombe DE, Netea MG, Erwig LP, Gow NAR, Brown AJP, Epitope Shaving Promotes Fungal Immune Evasion. mBio, 11(4):(2020) [pubmed]

submitter keyword: Candida albicans, secretome, LC-MSMS

Professor Alistair J P Brown
contact affiliation	MRC Centre for Medical Mycology University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
contact email	a.j.p.brown@exeter.ac.uk
lab head
David Stead
contact affiliation	University of Aberdeen
contact email	d.stead@abdn.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018044

PRIDE project URI

• PRIDE
  1. PXD018044
     1. Label: PRIDE project
     2. Name: Epitope shaving and fungal immune evasion – culture supernatants