PXD018041

PXD018041 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of human A549 Lung cancer cells harboring LKB1 mutations.
Description	STK11 (LKB1) missense somatic mutant isoforms promote tumor growth, motility and inflammation. Elucidating the contribution of somatic mutations to cancer is essential for personalized medicine. STK11 (LKB1) tumor suppressor appears to be inactivated in human cancer, however, somatic missense mutations also occur. Despite of our increased knowledge about LKB1 function, the role/s of these alterations in cancer are mostly unknown. Here, we investigated the contribution of four missense LKB1 somatic mutations in tumor biology. Three, out of the four mutants, lost their tumor suppressor capabilities and showed a deficient kinase activity. The remaining mutant conserved the enzymatic activity, but conferred an increased cell motility. Mechanistically, LKB1 mutants promoted the differential gene expression regulation of vesicle trafficking regulating molecules, adhesion molecules and cytokines, that correlated with the identified protein networks associated to the comparative secretome analysis. Notably, three mutant isoforms promoted tumor growth, and one of them induced inflammation and hemorrhagic tumors that correlated with the deregulated levels of cytokines. Altogether, our findings uncover oncogenic roles of LKB1 somatic mutations helping to understand their contribution to cancer.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_01:33:55.196.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesc Canals
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 02:48:29	ID requested
⏵ 1	2021-09-09 01:33:56	announced

Granado-Mart, í, nez P, Garcia-Ortega S, Gonz, á, lez-S, á, nchez E, McGrail K, Selgas R, Grueso J, Gil R, Naldaiz-Gastesi N, Rhodes AC, Hernandez-Losa J, Ferrer B, Canals F, Villanueva J, M, é, ndez O, Espinosa-Gil S, Lizcano JM, Mu, ñ, oz-Couselo E, Garc, í, a-Patos V, Recio JA, STK11 (LKB1) missense somatic mutant isoforms promote tumor growth, motility and inflammation. Commun Biol, 3(1):366(2020) [pubmed]

submitter keyword: Human

Lung Cancer

LKB1

LC-MSMS

Secretome

Juan A. Recio
contact affiliation	Biomedical Research in Melanoma-Animal Models and Cancer Laboratory- Vall d´Hebron Research Institute VHIR-Vall d’Hebron Hospital Barcelona-UAB 08035, Spain.
contact email	juan.recio@vhir.org
lab head
Francesc Canals
contact affiliation	Vall Hebron Institute of Oncology
contact email	fcanals@vhio.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018041

PRIDE project URI

• PRIDE
  1. PXD018041
     1. Label: PRIDE project
     2. Name: Secretome analysis of human A549 Lung cancer cells harboring LKB1 mutations.