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PXD018034 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMaturation kinetics of a multiprotein complex revealed by metabolic labeling
DescriptionThe project aims at computing in vivo co-assembly kinetics from metabolomic-inspired approaches. In short, we performed reciprocal pull-down experiments from complex cellular components at different time points following pulsed-SILAC metabolic labeling. We applied the method to 320 pairs of yeast nucleoporin (NUP) proteins constituting the ~ 50 MDa Nuclear Pore Complex (NPC). Our analysis reveals a hierarchical principle of the NPC biogenesis where individual subcomplexes form on a minute time-scale, followed by their co-assembly from central to peripheral subunits in a ~ 1 hour long maturation process.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLudovic Gillet
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-13 01:28:54ID requested
12020-10-16 01:19:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: yeast, nuclear pore complex, maturation kinetics, pulsed SILAC, data independent acquisition
Contact List
Evgeny Onischenko
contact affiliationDepartment of Biological Sciences, University of Bergen, Bergen, Norway
contact emailevgeny.onishchenko@uib.no
lab head
Ludovic Gillet
contact affiliationETH Zurich
contact emailgillet@imsb.biol.ethz.ch
dataset submitter
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