PXD018028

PXD018028 is an original dataset announced via ProteomeXchange.

Title	c-di-GMP-induced conformational dynamics of the c-di-GMP-binding protein A (CdbA) from Myxococcus xanthus
Description	The second messenger c-di-GMP has important functions in response to changing environmental and cellular cues in bacteria. Here, we report the identification of CdbA, a DNA binding protein of the ribbon-helix-helix family in Myxococcus xanthus, and show that it binds c-di-GMP in vitro. CdbA is essential for viability and its depletion caused defects in chromosome organization and segregation resulting in a block in cell division. CdbA binds multiple sites across the M. xanthus genome with moderate sequence specificity; however, its depletion only caused minor changes in transcription. C-di-GMP binding and DNA binding by CdbA are mutually exclusive and substitutions in the CdbA interfaces important for c-di-GMP binding not only abolished c-di-GMP binding but also DNA binding and rendered the mutant proteins non-functional in vivo. Our data are consistent with a model whereby CdbA functions as a nucleoid associated protein to organize the M. xanthus chromosome and the function of CdbA is modulated by c-di-GMP, thus, for the first time, establishing a link between c-di-GMP and chromosome organization and segregation.
HostingRepository	PRIDE
AnnounceDate	2020-04-16
AnnouncementXML	Submission_2020-07-14_23:12:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wieland Steinchen
SpeciesList	scientific name: Myxococcus xanthus (strain DK 1622); NCBI TaxID: 246197;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-12 08:20:51	ID requested
1	2020-04-16 06:28:29	announced
⏵ 2	2020-07-14 23:12:56	announced	2020-07-15: Updated publication reference for PubMed record(s): 32286293.

Skotnicka D, Steinchen W, Szadkowski D, Cadby IT, Lovering AL, Bange G, S, ø, gaard-Andersen L, CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus. Nat Commun, 11(1):1791(2020) [pubmed]

submitter keyword: HDX-MS, c-di-GMP, PLGS, DynamX

Gert Bange
contact affiliation	Philipps-University Marburg, Department of Chemistry & SYNMIKRO, Hans-Meerwein-Straße, 35043 Marburg, GERMANY
contact email	gert.bange@synmikro.uni-marburg.de
lab head
Wieland Steinchen
contact affiliation	Philipps-University Marburg, Department of Chemistry & SYNMIKRO, Hans-Meerwein-Straße, 35043 Marburg, GERMANY
contact email	wieland.steinchen@synmikro.uni-marburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018028
     1. Label: PRIDE project
     2. Name: c-di-GMP-induced conformational dynamics of the c-di-GMP-binding protein A (CdbA) from Myxococcus xanthus