PXD018025

PXD018025 is an original dataset announced via ProteomeXchange.

Title	Testing serum specificity for bacterial antigens in a mouse model of spontaneous colitis
Description	Identification of bacterial antigens within cecal bacterial lysate was performed by using serum antibodies from control and DC-LMP1/CD40 animals for immunoprecipitation followed by label-free liquid chromatography tandem mass spectrometry (LC-MS/MS). Serum antibodies were coupled to beads and incubated with cecal bacterial lysate to bind target proteins. Upon immunoprecipitation, on-beads digestion of proteins followed by LC-MS/MS was performed. The resulting peak intensities were used for intensity-based quantification (iBAQ). Proteins identified with a fold change > 2 and a p-value < 0.05 were considered for further analyses.
HostingRepository	PRIDE
AnnounceDate	2021-02-25
AnnouncementXML	Submission_2021-02-25_02:43:30.199.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Helicobacter hepaticus; NCBI TaxID: 32025;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-12 04:43:02	ID requested
1	2021-02-25 01:33:45	announced
⏵ 2	2021-02-25 02:43:31	announced	2021-02-25: Updated project metadata.

Friedrich V, Forn, é I, Matzek D, Ring D, Popper B, Jochum L, Spriewald S, Straub T, Imhof A, Krug A, Stecher B, Brocker T, is required for immune targeting of bacterial heat shock protein 60 and fatal colitis in mice. Gut Microbes, 13(1):1-20(2021) [pubmed]

10.1080/19490976.2021.1882928;

submitter keyword: spontaneous colitis, Helicobacter hepaticus

Prof. Thomas Brocker
contact affiliation	Institute for Immunology, BioMedical Center, Faculty of Medicine, LMU Munich, 82152 Munich, Germany
contact email	tbrocker@med.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD018025

PRIDE project URI

• PRIDE
  1. PXD018025
     1. Label: PRIDE project
     2. Name: Testing serum specificity for bacterial antigens in a mouse model of spontaneous colitis