PXD018007

PXD018007 is an original dataset announced via ProteomeXchange.

Title	tRNA binding to KEOPS human protein complex
Description	N6-theronylcarbomyl adenosine (t6A) is a universal tRNA posttranscriptional modification that promotes translation fidelity and is therefore required for the fitness of virtually all living cells. Kae1, the t6A modifying enzyme in eukaryotes and archea, resides within the KEOPS complex with three auxiliary subunits of unknown function namely Cgi121, Bud32 and Pcc1. Through biochemical and X-ray crystallographic analyses we show that Cgi121 functions to recruit substrate tRNA to KEOPS via its universal 3’-CCA tail. Incorporation of the Cgi121-tRNA structure into a composite model of KEOPS reveals an extended tRNA contact surface that surprisingly spans all four subunits. Comprehensive mutational and functional analyses validate the relevance of the extended tRNA-binding surface in vivo and in vivo. Together this work provides insight into the t6A catalytic cycle and its regulation through the cooperative action of all four KEOPS subunit through direct contacts with tRNA.
HostingRepository	PRIDE
AnnounceDate	2020-12-11
AnnouncementXML	Submission_2020-12-10_23:15:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dave Schriemer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-12 00:22:42	ID requested
⏵ 1	2020-12-10 23:15:27	announced

Beenstock J, Ona SM, Porat J, Orlicky S, Wan LCK, Ceccarelli DF, Maisonneuve P, Szilard RK, Yin Z, Setiaputra D, Mao DYL, Khan M, Raval S, Schriemer DC, Bayfield MA, Durocher D, Sicheri F, A substrate binding model for the KEOPS tRNA modifying complex. Nat Commun, 11(1):6233(2020) [pubmed]

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: tRNA, KEOPS, HX-MS

David Christopher Schriemer
contact affiliation	Dept. of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary Canada
contact email	dschriem@ucalgary.ca
lab head
Dave Schriemer
contact affiliation	Dept. Biochemistry and Molecular Biology, University of Calgary
contact email	dschriem@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018007

PRIDE project URI

• PRIDE
  1. PXD018007
     1. Label: PRIDE project
     2. Name: tRNA binding to KEOPS human protein complex