PXD017998

PXD017998 is an original dataset announced via ProteomeXchange.

Title	Changes in golden Syrian hamster HDL proteome caused by altered cholesteryl ester transfer protein expression
Description	Cholesteryl ester transfer protein (CETP) transfers cholesteryl ester (CE) and triglyceride (TG) between lipoproteins, which alters lipoprotein metabolism. Hamster and human CETPs have very different preferences for CE versus TG as substrate. To assess the impact of altering CETP’s substrate preference on lipoproteins in vivo, human CETP was expressed in hamsters (Mesocricetus auratus). Chow-fed hamsters received adenoviruses expressing no CETP (Ad-Null), hamster CETP (Ad-hamster CETP) or human CETP (Ad-human CETP). High density lipoproteins were isolated from hamster plasma 6 days after adenovirus injection by ultracentrifugation as the 1.063 - 1.21 g/ml density fraction. HDL proteins were precipitated with cold acetone and subjected to LC+MS/MS analysis
HostingRepository	PRIDE
AnnounceDate	2020-06-25
AnnouncementXML	Submission_2020-07-01_00:02:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017998
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ling Li
SpeciesList	scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 07:02:26	ID requested
1	2020-06-25 04:53:50	announced
⏵ 2	2020-07-01 00:02:39	announced	2020-07-01: Updated publication reference for PubMed record(s): 32591337.

Morton RE, Liu Y, The lipid transfer properties of CETP define the concentration and composition of plasma lipoproteins. J Lipid Res, 61(8):1168-1179(2020) [pubmed]

submitter keyword: hamster, plasma, high density lipoprotein, cholesteryl ester transfer protein, LC-MS/MS

Richard Morton
contact affiliation	Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic
contact email	MORTONR@ccf.org
lab head
Ling Li
contact affiliation	Cleveland Clinic
contact email	lil5@ccf.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017998
     1. Label: PRIDE project
     2. Name: Changes in golden Syrian hamster HDL proteome caused by altered cholesteryl ester transfer protein expression