PXD017990

PXD017990 is an original dataset announced via ProteomeXchange.

Title	Global proteome analysis of conditional sth1 mutant in Candida albicans
Description	Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. We addressed the global functions of RSC in C. albicans by analyzing the changes in the proteome of cells lacking the catalytic subunit Sth1 using DIA-MS (Data Independent Acquisition-Mass Spectrometry). For this purpose, we generated a conditional mutant harboring a doxycycline (dox)-repressible allele of STH1 (sth1∆/pTetO-STH1-MYC) and treated this mutant with dox for Sth1 depletion. To assess the effect of dox alone on gene expression, a near-isogenic sth1∆/STH1-MYC strain was also analysed.
HostingRepository	PRIDE
AnnounceDate	2020-11-11
AnnouncementXML	Submission_2020-11-11_09:27:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vinutha K B
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 05:34:09	ID requested
⏵ 1	2020-11-11 09:28:00	announced

Balachandra VK, Verma J, Shankar M, Tucey TM, Traven A, Schittenhelm RB, Ghosh SK, The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits. PLoS Genet, 16(11):e1009071(2020) [pubmed]

submitter keyword: DIA-MS, Sth1, RSC, Candida albicans

Santanu Kumar Ghosh
contact affiliation	Chromosome and Plasmid Segregation Lab, Department of Biosciences and Bioengineering, IIT Bombay Mumbai, India
contact email	santanughosh@iitb.ac.in
lab head
Vinutha K B
contact affiliation	IIT Bombay and Monash University
contact email	vinutha.kb@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017990
     1. Label: PRIDE project
     2. Name: Global proteome analysis of conditional sth1 mutant in Candida albicans